编码EIAV核输出因子蛋白Rev的cDNA克隆和序列测定  

Cloning and Sequencing of cDNA Equine Infectious Anemia Virus Rev Protein

作　　者：张宝山[1] 孙成群[1] 刘相冬[1] 刘永刚[1] 周家喜[1] 王凯波[1] 刘建华[1] 孔宪刚[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,哈尔滨150001

出　　处：《中国预防兽医学报》2000年第S1期11-,共1页Chinese Journal of Preventive Veterinary Medicine

摘　　要：ＥＩＡＶ在繁殖过程中涉及到多种转录因子的调节，其中Ｒｅｖ蛋白是病毒编码的转录后调节因子，决定了病毒复制由早期到晚期的过渡。Ｒｅｖ是非结构蛋白，由病毒基因组全长转录产物经多次拼接而成。本研究使用ＥＩＡＶ疫苗毒感染驴巨噬细胞培养物，在病毒繁殖早期提取细胞总ＲＮＡ，反转录后使用中国ＥＩＡＶ毒株特异引物扩增病毒基因组拼接产物。将扩增产物克隆后经过核苷酸序列分析，和与基因组全序列的比较，确定了编码Ｒｅｖ蛋白的病毒基因组转录产物的编码序列和拼接位点。Replication of EIAV is regulated by seveal transcription factors. Rev is encoded by multi-spliced genomic mRNA, which switches the virus gene expression from early stage to late stage. In this study, donkey macrophage culture was infected with donkey leukocyte attenuated EIAV; total cellular RNA was extracted from cells at the early stage of the infection. After reverse transcription directed by EIAV specific primer,polymerase chain reaction was performed. Then the amplified segment was cloned and...

关 键 词：EIAV核输出因子蛋白 CDNA克隆 序列测定 

分 类 号：S85[农业科学—兽医学]