编码EIAV反式激活蛋白Tat的cDNA克隆和序列测定  

Cloning and Sequencing of cDNA Encoding Equine Infectious Anemia Virus Trans-activating Protein

作　　者：张宝山[1] 孙成群[1] 刘相冬[1] 刘永刚[1] 周家喜[1] 王凯波[1] 刘建华[1] 孔宪刚[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室,哈尔滨150001

出　　处：《中国预防兽医学报》2000年第S1期93-,共1页Chinese Journal of Preventive Veterinary Medicine

摘　　要：ＥＩＡＶ在繁殖过程中的转录涉及到多种因子的调节，其中ＴＡＴ蛋白是病毒编码的反式激活因子，是病毒复制必须成分。ＴＡＴ是非结构蛋白，由病毒基因组全长转录产物经多次拼接而成。本研究使用ＥＩＡＶ疫苗毒感染驴巨噬细胞培养物，在病毒增殖早期提取细胞总ＲＮＡ，反转录后使用中国ＥＩＡＶ毒株特异引物扩增病毒基因组拼接产物。将扩增产物克隆后经过核苷酸序列分析，和与基因组全序列的比较。确定了编码ＥＩＡＶ反式激活蛋白的转录产物及阅读框架及转录后拼接位点，研究发现至少有两种拼接产物编码ＴＡＴ。The transcription of EIAV was regulated by several viral and cellular factors; TAT is a transactivating protein encoded by the virus genome and it is necessary for virus replication. mRNAs encoding TAT were spliced from full length viral transcript in the host cell. In this study, donkey macrophage culture was infected with donkey leukocyte attenuated EIAV. At the early stage of virus replication total RNA was extracted from infected cells. After reverse transcription, polymerase chain reaction was per...

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分 类 号：S85[农业科学—兽医学]