白腐菌液体培养产生木质纤维素降解酶的研究  

STUDIES ON WHITE ROT Fungi. PRODUCING LIGNINOCELLULOSE-DEGRADING ENZYMES IN LIQUID STATE FERMENTATION

作　　者：谢君[1] 任路[1] 李维[1] 孙迅[2] 张义正[1] 

机构地区：[1]四川大学生命科学学院分子生物学实验室,成都610064 [2]山东菏泽师范学院生物系,山东菏泽274015

出　　处：《四川大学学报（自然科学版）》2000年第S1期161-166,共6页Journal of Sichuan University(Natural Science Edition)

摘　　要：选用 4 5枚白腐真菌 ,首先根据RB亮蓝变色反应 ,初筛出 6枚菌株 .采用正交实验确定了这 6枚菌株的最佳液体产酶培养基 ,讨论了它们产木质素降解酶的行为 .根据其产酶的特性 ,从中筛出 2枚在液体培养基中产酶能力最强且产酶较快的菌株 .结果表明 ,侧耳sp2和粗毛栓菌在液体培养中具有很强的产酶能力且产酶较快 ,且首先降解木质素 ,是生物制浆中原料预处理的优良菌株 ,具有一定的应用前景 .white rot fungi. Were used in this essay. Six of them were screened with Remazol blue(RB) reactions according to their capability and speed of RB faded. The optimal liquid media for the enzymes producing were defined by the orthogonal trial, and the behavior of their enzyme producing was discussed. According to their ability of enzyme producing and time of enzyme producing peak, 2 strains were screened. The work indicated that the en...

关 键 词：白腐真菌 菌株筛选 液体培养 产酶能力 木质纤维素降解 

分 类 号：O629[理学—有机化学]