叶酸诱导肝癌细胞凋亡及其分子机制的研究  

STUDY ON APOPTOSIS OF HEPATOCARCINOMA CELLS INDUCED BY FOLIC ACID AND ITS MOLECULAR MECHNISM

作　　者：袁淑兰[1] 王艳萍[1] 李良平[1] 陈晓禾[1] 宋毅[1] 杨燕[1] 

机构地区：[1]华西医科大学附一院肿瘤中心肿瘤研究所,成都610041

出　　处：《四川大学学报（自然科学版）》2000年第S1期259-263,共5页Journal of Sichuan University(Natural Science Edition)

摘　　要：应用叶酸连续处理体外培养的人肝癌细胞 (SMMC 772 1) ,4d后光镜及电镜观察细胞形态改变 ,通过细胞生长曲线测定细胞生长及增殖情况 ,流式细胞仪检测细胞周期分布、细胞凋亡指数及凋亡相关基因及其表达的改变 ,以探讨叶酸诱导肝癌细胞凋亡的作用及其分子机制 .结果显示 ,10 μg/mL叶酸可诱导SMMC 772 1细胞出现凋亡特征性形态改变 ,细胞凋亡指数为 16 .8% (空白对照组为 6 .9% ) ,S期细胞减少、细胞生长和增殖被明显抑制 ,且凋亡相关基因fas和p53表达降低 ,而bcl 2和TGF β1表达增加 .研究提示 ,叶酸具有诱导人肝癌细胞凋亡的作用 ,其分子机制可能与调控细胞凋亡相关基因的表达有关 .Human hepatocarcinoma SMM C 7721 cells cultured in vitro were treated wit h folic acid(FA) for 4 days. The morphology of the c ells were observed by microscopy. The cell growth and proliferation were measured cell growth curve. The cell cycle,apoptosis index(AI) and the expression of apoptosi s associated gene were analysed by flow cytometry. The res ults showed that 10 μg/mL FA could induce apoptosis of SM MC 7721 cells. The morphologic ...

关 键 词：叶酸 肝癌细胞 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]