弓形虫可溶性抗原免疫-PCR检测技术的建立  

Establishment of Immuno-PCR Technique for Detection of Toxoplasma Dissoluble Antigens

作　　者：郑兰艳[1] 王海鹏[1] 

机构地区：[1]中国医科大学基础医学院病原生物学教研室,沈阳110001

出　　处：《中国医科大学学报》2000年第S1期27-29,共3页Journal of China Medical University

摘　　要：目的 :建立一种新的特异性强 ,敏感性高的弓形虫可溶性抗原检测方法。方法 :用链霉亲和素将生物素化的二抗和生物素化的 DNA偶联起来 ,通过 PCR扩增标记的 DNA检测固定的抗原 ,建立免疫 - PCR技术。通过选择最佳的链霉亲和素浓度和生物素化 DNA的浓度 ,控制了非特异性扩增。结果 :免疫 - PCR检测抗原最低限为 1ng/m l,EL ISA法检测抗原的最低限为 0 .8μg/ml,敏感性约为 EL ISA的 80 0倍。结论 :免疫 - PCR是一种特异性强 ,敏感性高的弓形虫可溶性抗原检测方法。Objective:The purpose of this study was to establish a highly specific and sensitive Immuno PCR method for the detection of Toxoplasma dissoluble antigens(DBAg). Methods:Free streptavidin was used to attach a biotinylated DNA to the biotinylated second antibody, Antigen which bound with antibodies was detected by amplication of the DNA label, thus Toxoplasma dissoluble antigens Immuno PCR assay was established. We controlled the nonspecific amplification using the optimal concentration of DNA label and st...

关 键 词：免疫-聚合酶链反应 可溶性抗原 弓形虫 

分 类 号：R378[医药卫生—病原生物学]