腺病毒载体介导的CD自杀基因体外抑瘤效果观察  

In Vitro Tumor-killing Effect of Suicide CDGene Induced by Adenovirus Vector

作　　者：高军[1] 崔龙[2] 龙建秋[1] 谷爱梅[1] 李茂[1] 戴观荣[2] 潘卫[2] 曹广文[2] 

机构地区：[1]海军医学研究所,上海200433 [2]第二军医大学

出　　处：《海军医学杂志》2000年第3期218-221,共4页Journal of Navy Medicine

基　　金：国家自然基金资助!项目号 3960 0 1 72

摘　　要：目的 :构建含CD基因的腺病毒载体pAd -CD ,进行肿瘤的自杀基因治疗。方法 :分别构建了含CD基因和LacZ基因的重组病毒质粒载体 ,同质粒pJM17共转染 2 93细胞同源重组后包装为感染性腺病毒颗粒 ,经滴度测定后 ,体外分别感染小鼠前胃癌肿瘤细胞MFC。结果 :感染Ad -LacZ的细胞可被X - gal染成蓝色 ,感染Ad -CD的细胞予前药 5 -FC可见肿瘤细胞杀伤效果明显。结论 :腺病毒载体可有效地转CD和LacZ基因进入肿瘤细胞 ,CD基因作为自杀基因进行体外肿瘤治疗效果明显 ,本实验构建的新载体及结果为下一步应用于体内肿瘤基因治疗研究提供了基础。Objective:Construction of an adenovirus—pAd-CD containing bacterial cytosine deaminase was used for tumor suicide gene therapy. Meth od:We constru cted a recombinant adenoviral vector carrying CD or LacZ. The infectious adenovi ruse expressing CD were produced by 293 packaging cells transfected by the r ecom binant plasmid and plasmid pJM17. After titer of the adenovirus， they were us ed to infect mouse tumor cells—MFC in vitro. Result:Cel ls infected by Ad-L acZ were dyed blue by X-gal. Cells infected by Ad-CD were obviously killed after administration of 5-FC. Conclusion:Adenoviral vector can efficiently transfect LacZ and CD gene to tumor cells. CD as a suicide gene is able to kill tumor cel l in vitro. The new adenoviral vector constructed in our lab and the researc h re sults provide sound basis for future application of tumor gene therapy in vivo.

关 键 词：CD基因 腺病毒 肿瘤 

分 类 号：R[医药卫生]