hbFGF基因转染骨髓间充质干细胞促进种植体周骨界面形成的研究  

Research on promoting bone regeneration around dental implants with bone marrow mesenchymal stem cells transfected by human bFGF gene

作　　者：谭震[1] 陈超[1] 宫苹[1] 欧国敏[1] 汪成孝[2] 魏娜[1] 唐华[1] 廖大鹏[1] 

机构地区：[1]四川大学华西口腔医院种植中心,610041 [2]四川省干细胞研究所

出　　处：《中国口腔种植学杂志》2009年第2期123-124,共2页Chinese Journal of Oral Implantology

摘　　要：目的:本研究通过构建质粒载体pDC316bFGF-IREs-EGFP,将bFGF基因转移至MSCs中,再移植到种植体周围,为寻找一种新的促进种植体周骨界面形成的方法提供实验依据。方法:采用密度梯度离心法分离培养犬BM-MSCs,通过细胞表面标记和分化能力进行鉴定。将重组质粒pDC316bFGF-IREs-EGFP通过脂质体Lipofectamine2000介导转染犬骨髓源MSCs。再将其与藻酸钙凝胶复合移植到种植体周围,4周后通过大体、放射线、Micro-CT及组织学观察缺损再生情况。结果:结果显示实验组种植体周围骨组织的再生速度明显快于未转染bFGF的MSCs移植组。讨论和结论:pDC316bFGF-IREs-EGFP转染的MSCs可以提高骨形成的效率,具有潜在的临床应用价值。This study is undertaken to supply an experimental foundation for seeking new biologic means for the treatment of periodontitis and peri-implantitis.MSCs of Canines were isolated,cultured and purified by the methods of density gradient centrifugation,and the cells were identified by surface marker and differentiating capacity.Combined with calcium alginate gel,MSCs transfected by recombinant plasmid pDC316bFGF-IREs-EGFP were transplanted to space around dental implant.After four weeks,the defect regeneration was evaluated by clinical examination,X-ray,Micro-CT and histological observation.Animal experiment proved regenerating speed of periodontal bone tissue in groups transplanted by MSCs with bFGF was higher than those transplanted by MSCs without bFGF.bFGF overexpression mediated by recombinant plasmid pDC316bFGF-IREs-EGFP could accelerate bone regeneration.It might have a potential value of clinical application.Kyewords] bone marrow mesenchymal stem cells;gene therapy;Plasmid;basic fibroblast growth factors;bone regeneration

关 键 词：骨髓间充质干细胞(BMSC) 质粒 基因治疗 碱性成纤维细胞生长因子(bFGF) 骨组织再生 

分 类 号：R783[医药卫生—口腔医学]