大鼠肠道缺血预适应对缺血再灌注损伤的保护作用  

Protectine effect of ischemic preconditioning on the small intestine ischemic-reperfusion injury in rat

作　　者：张培建[1] 王懋莉[1] 陶立德[1] 梁四海[1] 李勇[1] 周斌[1] 金成[1] 卜平[1] 

机构地区：[1]扬州大学医学院,江苏扬州225001

出　　处：《扬州大学学报（农业与生命科学版）》2009年第2期17-20,共4页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：江苏省社会发展科技计划项目(BS2005038);江苏省卫生厅面上科研项目(H200770)

摘　　要：采用清洁级SD大鼠24只,随机均分为3组,S组为正常对照组,A组为缺血再灌注损伤组,B组为肠道缺血预适应组。A、B组大鼠血流再灌注后1 h取Treitz韧带下5 cm空肠作为标本,测定肠道髓过氧化物酶(MPO)活性与丙二醛(MDA)含量,光镜观察小肠组织病理变化,黏膜湿重、微绒毛长度及直径,门静脉血测定血清超氧化物歧化酶(SOD)和一氧化氮(NO)含量,研究大鼠肠道缺血预适应对肠道缺血再灌注损伤的影响。结果表明:A组小肠MPO活性和MDA含量明显高于S组、B组,S组和B组之间差异不显著;A组血清SOD活性、NO含量比S组和B组显著降低;S组和B组之间差异不显著。A组空肠黏膜湿重、绒毛长度和直径显著低于S组与B组。光镜下见A组空肠形态学损伤较B组与S组重。证明大鼠肠道缺血预适应对肠道缺血再灌注损伤具有保护作用。To investigate the effect on small intestine mediated by the ischemic preconditioning after ischemic-reperfusion injury.SD rats were randomly assigned into the following study groups,S group: sham operation group,n=8;A group: ischemic-reperfusion injury group,n=8;B group: ischemic preconditioning on the rat s small intestine plus ischemic-reperfusion injury group,n=8.When the intestine was reperfused for 1 h,the MDA and MPO of the intestine were determined.The SOD and NO were determined in serum as well.The...

关 键 词：大鼠 缺血预适应 肠道黏膜屏障 

分 类 号：R574[医药卫生—消化系统]