Surveillance of Kanamycin Resistance to Escherichia coll from Swine by Digoxigenin-labled Plasmid Probe  

作　　者：YANG Han-chun, ZHAO Jing, LIU Jin-hua, ZHA Zhen-lin and CHEN Yan-hong( College of Veterinary Medicine, China Agricultural University, Beijing 100094 ,P.R. China ) 

出　　处：《Agricultural Sciences in China》2002年第5期581-584,共4页中国农业科学（英文版）

摘　　要：A 4.34kb EcoR I fragment of kanamycin resistance plasmid from pET - 9a was purified by a DNA purification kit. The fragment was labeled with digoxigenin-dUTP with a commercial kit. A dot-blot hybridization and a colony hybridization test with the probe were successfully developed for the surveillance of Kanamycin resistance to E.coli from swine. It was shown that the methods obtained 100% concordance in a positive tate. It was indicated that the method was available for the surveillance of kanamycin resistance to ? coli from swine.A 4.34kb EcoR I fragment of kanamycin resistance plasmid from pET - 9a was purified by a DNA purification kit. The fragment was labeled with digoxigenin-dUTP with a commercial kit. A dot-blot hybridization and a colony hybridization test with the probe were successfully developed for the surveillance of Kanamycin resistance to E.coli from swine. It was shown that the methods obtained 100% concordance in a positive tate. It was indicated that the method was available for the surveillance of kanamycin resistance to ? coli from swine.

关 键 词：DIGOXIGENIN Plasmid probe E.coli Kanamycin resistance SURVEILLANCE 

分 类 号：S852.62[农业科学—基础兽医学]