云南高校图书馆联盟文献共享服务平台- DIGOXIGENIN

DIGOXIGENIN: 作品数：36被引量：79H指数：5; 导出分析报告; 相关领域：医药卫生更多>>; 相关作者：崔治中张立农肖雪君刘晓文潘志明更多>>; 相关机构：福建医学院江苏农学院中国农业大学扬州大学更多>>; 相关期刊：《Journal of Pharmaceutical Analysis》《广西医学》《内蒙古大学学报（自然科学版）》《生物技术》更多>>; 相关基金：国家自然科学基金广东省自然科学基金河南省科技攻关计划江苏省科委自然科学基金更多>>

应用多重Digoxigenin标记探针在核酸斑点杂交检测中识别Ⅰ群禽腺病毒的感染被引量：2: 《中国兽医学报》2020年第12期2305-2310,共6页侯力丹张亚文苏奇刘丹毛娅卿王嘉黄小洁赵鹏李俊平杨汉春; 国家重点研发计划资助项目(2016YFD0500800,2016 YFD0500809)。; 禽腺病毒(fowl adenovirus,FAdV)是鸡群中常见的免疫抑制性病毒,其中Ⅰ群FAdV有12个血清型(FAdV1-8a,8b-FAdV11),这为Ⅰ群FAdV检测带来了挑战。为开发一种可以同时检测Ⅰ群FAdV的Digoxigenin标记核酸探针或者其组合,本研究针对Ⅰ群FAdV...; 关键词：Ⅰ群禽腺病毒 hexon基因 DIGOXIGENIN 核酸斑点杂交

A Study of the Distribution of Apple stem pitting virus in Tissues of Pear Tree Using In Situ Hybridization and In Situ RT-PCR被引量：2: 《Agricultural Sciences in China》2009年第11期1351-1359,共9页ZHAO Ying LIU Na NIU Jian-xin; supported by the National Natural Science Foundation of China (30360066);the National Key Technologies R&D Program of China (2003BA546C);the Foundation Science and Technology Commission Xinjiang Production and Construction Corps,China(NKB02SDXNK01SW); To understand the distribution of Apple stem pitting virus （ASPV） in tissues of pear tree and provide directly theorical and technical support for shoot tips detoxication, leaves and shoot tips of Korla pear were us...; 关键词：Korla pear Apple stem pitting virus cDNA probe DIGOXIGENIN in situ PCR

Dot-Blot Hybridization for Detection of Five Cucurbit Viruses by Digoxigenin-Labelled cDNA Probes被引量：3: 《Agricultural Sciences in China》2007年第12期1450-1455,共6页MENG Juan GU Qin-sheng LIN Shi-ming PENG Bin LIU Li-feng TIAN Yan-ping LI Li; Dot-blot hybridization was applied in this paper to detect five viruses infecting cucurbitaceous crops, Zuccini yellow mosaic virus （ZYMV）, Watermelon mosaic virus （WMV）, Cucumber mosaic virus （CMV）, Papaya ring...; 关键词：PCR digoxigenin-labelled cDNA probe dot-blot hybridization ZYMV WMV CMV PRSV-W SqMV

Localization of Avian Influenza Virus in Formalin-Fixed, Paraffin-Embedded Chicken Tissues by In Situ Hybridization: 《Agricultural Sciences in China》2005年第12期931-936,共6页ZHANG Wan-po GU Chang-qin BI Ding-ren SONG Nian-hua CHENG Guo-fu; In this study, in situ hybridization （ISH） was developed to detect avian influenza＇virus （AIV） in Madin-Darby canine kidney （MDCK） cells and formalin-fixed, paraffin-embedded chicken tissues. A cDNA probe corre...; 关键词：In situ hybridization Avian influenza virus cDNA probe DIGOXIGENIN

Surveillance of Kanamycin Resistance to Escherichia coll from Swine by Digoxigenin-labled Plasmid Probe: 《Agricultural Sciences in China》2002年第5期581-584,共4页YANG Han-chun, ZHAO Jing, LIU Jin-hua, ZHA Zhen-lin and CHEN Yan-hong( College of Veterinary Medicine, China Agricultural University, Beijing 100094 ,P.R. China ); A 4.34kb EcoR I fragment of kanamycin resistance plasmid from pET - 9a was purified by a DNA purification kit. The fragment was labeled with digoxigenin-dUTP with a commercial kit. A dot-blot hybridization and a colon...; 关键词：DIGOXIGENIN Plasmid probe E.coli Kanamycin resistance SURVEILLANCE

Expression of exon 13 from the Ki-67 gene in human cells and tissues by digoxigenin-labelled mRNA in situ hybridization被引量：2: 《Chinese Medical Journal》2001年第1期48-53,共6页吴育连彭承宏沈宏伟; boththeNationalNaturalScienceFoundationofChina (No 396 0 0 14 1)andtheNaturalScienceFoundationofZhejiangProvince (No 396 498; OBJECTIVE: To get insight on the regulatory mechanism of Ki-67 gene expression in malignant cell cycle. METHODS: Non-radioactive in situ hybridization (ISH) was undertaken, combined with immunohistochemistry to study ...; 关键词：EXONS In Situ Hybridization DIGOXIGENIN Hela Cells Humans Immunohistochemistry Ki-67 Antigen RNA Messenger Research Support Non-U.S. Gov't

Hepatitis C virus may infect extrahepatic tissues in patients with hepatitis C被引量：3: 《World Journal of Gastroenterology》2000年第6期805-811,共7页Fu Ming Yan An Shen Chen Fei Hao Xi Ping Zhao Chang Hai Gu Li Bin Zhao Dong Liang Yang Lian Jie Hao; the Medical and Health Sciences Foundation of Chinese PLA,No.98D066; AIM To explore the status of extrahepatichepatitis C virus(HCV)infection and replicationin hepatitis C patients,and its potentialimplication in HCV infection and pathogenicity.METHODS By reverse-transcriptase poly-mer...; 关键词：HEPATITIS C virus HEPATITIS C ANTIGENS in situ hybridization immunohistochemistry RNA POLYMERASE chain reaction antibodies monoclonal DIGOXIGENIN

应用Digoxigenin标记的cDNA探针检测香蕉束顶病毒被引量：6: 《内蒙古大学学报（自然科学版）》1999年第3期367-371,共5页孟清曹先维张彤; 广东省自然科学基金; 用非放射性的Ｄｉｇｏｘｉｇｅｎｉｎ标记的１１－ｄＵＴＰ取代３２Ｐ标记的ｄＡＴＰ或ｄＣＴＰ，标记克隆的ＢＢＴＶｃＤＮＡ－１０．５ｋｂｃＤＮＡ，制备成探针．通过核酸斑点杂交对粗提取的ＢＢＴＶ，ＢＢＴＶ－ＤＮＡ，感染ＢＢＴ...; 关键词：香蕉束顶病毒 CDNA探针非放射性检测病毒

用 Digoxigenin 标记核酸探针检测 EDS-76 病毒核酸的研究被引量：4: 《中国畜禽传染病》1998年第1期40-42,共3页孙刚潘兴广刘尚高; 用ＥＤＳ７６病毒标准ＡＶ１２７株和分离株（Ｂ９６株）感染鸭胚，提取病毒核酸，分别经ＥｃｏＲｌ和Ｐｓｔｌ双酶切，获得图形和大小相似的酶切片段。分别进行ＥｃｏＲｌ和Ｐｓｔｌ单酶切，也得到相似结果。病毒ＤＮＡ经ＥｃｏＲｌ...; 关键词：减蛋综合症病毒核酸探针鸡病

RT-PCR和Digoxigenin标记的核酸探针检测鸡传染性支气管炎病毒试验研究被引量：2: 《河南农业大学学报》1997年第4期304-309,共6页王泽霖王丽阎若潜刘素云菅复春; 河南省科技攻关资助; 用２对已发表的引物和１对自行设计的引物对同一ＩＢＶＨ１２０株进行ＲＴ－ＰＣＲ，分别获得了Ｓ１基因上与引物设计相一致的１７２０ｂｐ，２２８ｂｐ，６０２ｂｐ，的扩增片段。用自行设计的引物对７个毒株（Ｈ１２０，Ｈ５２，Ｍ４...; 关键词：传染性支气管炎病毒鸡病检测核酸探针

DIGOXIGENIN