自身免疫性肝炎和原发性胆汁性肝硬化患者肝组织和外周血单个核细胞Fas及FasL的检测  

Detection of Fas and FasL in liver tissues and peripheral blood mononuclear cell of patients with autoimmune hepatitis or primary biliary cirrhosis

作　　者：刘妍[1] 闫惠平[1] 张立洁[1] 檀玉芬[1] 冯霞[1] 马冬梅[1] 张欣[1] 李伟华[1] 

机构地区：[1]首都医科大学附属北京佑安医院重点实验室,北京市100069

出　　处：《中华实验和临床感染病杂志（电子版）》2008年第2期39-43,共5页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：国家自然科学基金资助项目(30571723);北京市自然科学基金资助项目(7062033)

摘　　要：目的观察自身免疫性肝炎(AIH)和原发性胆汁性肝硬化(PBC)患者肝组织和外周血单个核细胞(PBMC)Fas、FasL的表达,探讨细胞凋亡在自身免疫性肝病(AILD)发病机制中的作用。方法采用免疫组织化学技术对10例AIH、20例PBC患者肝组织和PBMC中Fas、FasL进行检测,正常对照为10例健康献血者。结果AIH患者PBMC中Fas、FasL检出率分别为100%和90%,Fas、FasL平均阳性细胞率分别为78%和65%;PBC患者PBMC中Fas、FasL检出率均为90%,平均阳性细胞率分别为62%和59%。在肝组织中Fas、FasL表达于肝细胞和淋巴细胞:9例AIHFas阳性颗粒见于肝细胞浆,其中1例胞膜、胞浆同时阳性;8例FasL阳性颗粒表达于肝细胞浆;Fas、FasL阳性肝细胞多聚集于汇管区周围。同时在汇管区周围浸润的淋巴细胞中也可见Fas或FasL的阳性表达。17例PBC患者Fas阳性颗粒表达于肝细胞浆,其中4例胞膜、胞浆同时阳性;16例PBCFasL阳性,见于肝细胞浆,在肝组织中浸润的淋巴细胞浆中Fas、FasL均有表达。AIH和PBC患者Fas、FasL表达显著高于正常对照。结论AIH和PBC患者的Fas、FasL表达增加,细胞凋亡可能在AIH和PBC的发病机制中起重要作用。Objective To explore the role of apoptosis in pathogenesis of autoimmune hepatitis(AIH) and primary biliary cirrhosis(PBC) through observing expression of Fas and FasL in liver tissues and peripheral blood mononuclear cell(PBMC) of patients with AIH or PBC.Methods Ten patients with AIH and 20 patients with PBC were enrolled.And 10 healthy people were chosen as normal control.Immunohistochemistry was used to detect Fas and FasL in liver tissues and PBMC.Results Ten cases found Fas and 9 cases found FasL in PBMC of 10 patients with AIH,and the average percent of positive cells for Fas and FasL were 78% and 65%,respectively.Nine of 10 PBC cases detected Fas and FasL in their PBMC,the average percent of positive cells of them were 62% and 59%,respectively.Fas and FasL were located in hepatocytes and lymphocytes in liver tissues.In 9/10 AIH cases,Fas was found in cytoplasm,one patient in cytoplasm and cell membrane of hepatocytes.FasL in 8 cases were located in cytoplasm.The distribution of Fas and FasL positive hepatocytes were around port in liver.Most of the infiltrating lymphocytes were aggregated in port.In 17/20 PBC patients Fas were located in cytoplasm and 4 in cytoplasm and cell membrane.FasL in 16 cases were loaded in the cytoplasm of hepatocytes.The expressions of Fas and FasL in AIH and PBC were higher than healthy people.Conclusions The expressions of Fas and FasL were higher in AIH and PBC than normal control,and apoptosis may play an important role in the pathogenesis of AIH and PBC.

关 键 词：自身免疫性肝炎 原发性胆汁性肝硬化 FAS FASL 

分 类 号：R575.1[医药卫生—消化系统] R575.2[医药卫生—内科学]