Gene expression profiling in porcine mammary gland during lactation and identification of breed- and developmental-stage-specific genes  

作　　者：SU Zhixi1, DONG Xinjiao2, ZHANG Bing1, ZENG Yanwu1, FU Yan1, YU Jun1,3 & HU Songnian1,3 1. James D. Watson Institute of Genome Sciences, Zhejiang University, Hangzhou 310008, China 2. School of Life and Environmental Sciences, Wenzhou Normal College, Wenzhou 325027, China 3. Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, China 

出　　处：《Science China(Life Sciences)》2006年第1期26-36,共11页中国科学（生命科学英文版）

摘　　要：A total of 28941 ESTs were sequenced from five 5′-directed non-normalized cDNA libraries, which were assembled into 2212 contigs; 5642 singlets using CAP3. These sequences were annotated; clustered into 6857 unique genes, 2072 of which having no functional annotations were considered as novel genes. These genes were further classified into Gene Ontology categories. By comparing the expression profiles, we identified some breed-and developmental-stage-specific gene groups. These genes may be relative to reproductive performance or play important roles in milk synthesis, secretion; mammary involution. The unknown EST sequences; expression profiles at different developmental stages; breeds are very important resources for further research.A total of 28941 ESTs were sequenced from five 5?-directed non-normalized cDNA li-braries, which were assembled into 2212 contigs and 5642 singlets using CAP3. These sequences were annotated and clustered into 6857 unique genes, 2072 of which having no functional annotations were considered as novel genes. These genes were further classified into Gene Ontology categories. By comparing the expression profiles, we identified some breed- and developmental-stage-specific gene groups. These genes may be relative to reproductive performance or play important roles in milk synthesis, secretion and mammary involution. The unknown EST sequences and expression profiles at different developmental stages and breeds are very important resources for further research.

关 键 词：EXPRESSED sequence tags  expression profile  domestic pig  MAMMARY gland. 

分 类 号：S828[农业科学—畜牧学]