人参皂甙单体Rh_2对K562细胞增殖和凋亡作用的实验研究  

Effects of ginsenoside-Rh_2 on proliferation and apoptosis for K562 cell line

作　　者：袁忠海[1] 侯毅鞠[1] 李艳[1] 郭素红[1] 郝峰[1] 李正祎[1] 鞠晓红[1] 

机构地区：[1]吉林医药学院,吉林吉林132013

出　　处：《吉林医药学院学报》2006年第4期193-195,共3页Journal of Jilin Medical University

摘　　要：目的研究人参皂甙单体-Rh2(GS-Rh2)对人K562细胞增殖和凋亡作用的影响。方法采用MTT比色法观察GS-Rh2对体外培养的K562细胞生长的抑制作用;在光学显微镜下观察不同浓度GS-Rh2对K562细胞的凋亡作用及计数凋亡率(AR)。结果MTT比色法测定显示,细胞经GS-Rh2作用后生长受抑制,呈剂量依赖性和时间依赖性,半数抑制浓度(IC50)为25.8μg/m l。结论GS-Rh2能抑制体外培养的K562细胞增殖并诱导其凋亡。Objective To study effect of ginsenosideRh_2(GS-Rh_2) on proliferation and apoptosis of human erythroleukemia cell line K562.Methods Cell growth inhibition was evaluated by MTT colorimetric assay.Apoptotic cells were determined by light microscope and apoptosis rate(AR) was counted.Results MTT colorimetric assay revealed that the inhibition of GS-Rh_2 to the growth of K562 cells was in a dose-and time-dependent manner,50% inhibition concentration(IC_(50)) value was 25.8μg/ml.GS-Rh_2 could induce apoptosis on K562 cells.Conclusion GS-Rh_2 exhibited growth inhibition effect and induced apoptosis on K562 cell line in vitro.

关 键 词：人参皂甙-Rh2 K562细胞 增殖 凋亡 

分 类 号：R735.7[医药卫生—肿瘤] R285.5[医药卫生—临床医学]