CD14-159C/T基因多态性对CD14表达及炎症介质平衡的影响  

Effect of CD14 genomic polymorphism on CD14 expression and inflammatory cytokine balance

作　　者：蔺静[1] 咸力明[2] 姚咏明[2] 侯晓霞[2] 于燕[2] 董宁[2] 盛志勇[2] 

机构地区：[1]解放军总医院第一附属医院全军烧伤研究所 [2]Burns Institute, First Hospital Affiliated to the PLA General Hospital (formerly 304th Hospital)

出　　处：《中国急救复苏与灾害医学杂志》2006年第1期32-35,54,共5页China Journal of Emergency Resuscitation and Disaster Medicine

基　　金：国家重点基础研究发展规划项目(973项目;2005CB522602);国家杰出青年科学基金项目(30125020);北京市科技计划重大项目(H020920020530)

摘　　要：目的:探讨CD14基因启动子-159C/T基因多态性对全血培养CD14表达及促炎/抗炎细胞因子平衡的影响。方法:采集118例健康献血员全血标本,运用聚合酶链反应(PCR)及限制性内切酶HaeIII对PCR产物的消化作用检测CD14基因多态性。采用全血细胞培养模型检测内毒素(LPS)刺激前后CD14mRNA表达、可溶性CD14(sCD14)浓度的变化以及LPS对促炎细胞因子TNF-α、IL-6及抗炎细胞因子IL-10的诱生水平的影响。结果:LPS刺激后,基因型TT与TC白细胞中CD14mRNA的表达及上清sCD14浓度均显著高于CC纯合子(P<0.05或0.01)。TT纯合子促炎细胞因子TNF-α的诱生水平明显高于基因型TC、CC,而抗炎细胞因子IL-10的诱生水平明显低于后两者(P<0.05或P<0.01)。T等位基因携带者的IL-6诱生水平则均显著高于CC纯合子(P<0.01)。结论:内毒素受体CD14-159C/T基因多态性对全血培养CD14的表达及释放产生明显影响,并与内毒素刺激后促炎/抗炎细胞因子平衡密切相关。Objective:To investigate whether -159C/T promoter polymorphism of the CD14 gene influences the CD14 expression as well as inflammatory cytokine balance. Methods:118 healthy human blood donors were included in the present study. The CD14 gene polymorphism was determined by polymerase chain reaction and subsequent HaeIII restriction enzyme digestion of the polymerase chain reaction products. CD14 mRNA expression and soluble CD14 levels were measured using a whole blood cell culture model with or without lipopolysaccharide (LPS) stimulation. TNF-α, IL-6 and IL-10 production induced by LPS were also measured by commercial available ELISA kits. Results:Among the 118 individuals, there were 40 subjects homozygous for the T allele (TT), 62 were heterozygous (CT), and 16 had the genotype CC. After LPS stimulation, the CD14 mRNA expression in leukocytes and soluble CD14 levels in supernatant were significantly higher in TT homozygotes and carriers of the genotype TC compared with individuals homozygous for the C allele (P<0.05 or 0.01). In addition, TNF-α production of TT homozygote were markedly higher than those of the TC and CC genetypes, while IL-10 production were the lowest of the three (P<0.05 or P<0.01). IL-6 production of the T allel carriers were both higher than CC homozygotes (P<0.01). Conclusion:The -159C/T promoter polymorphism of the LPS receptor CD14 might influence the CD14 expression as well as release in whole blood culture, and it appears to be associated with the balance between pro- and anti-inflammatory cytokines.

关 键 词：CD14 基因多态性 全血培养 内毒素 促炎/抗炎细胞因子 

分 类 号：R[医药卫生]