水稻内生联合固氮细菌阴沟肠杆菌营养及其配方的研究  

Nutrient requirement and media of associative nitrogen-fixing bacterium Enterobacter cloace in rice plants

作　　者：陈夕军[1] 何平平[1] 童蕴慧[1] 纪兆林[1] 徐敬友[1] 

机构地区：[1]扬州大学园艺与植物保护学院,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2009年第3期91-94,共4页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(30571243)

摘　　要：阴沟肠杆菌(Enterobacter cloacae)J115和G161是2株具高固氮活性的水稻内生细菌。经研究发现,2株固氮细菌的营养要求基本相似,具体配方为:2%葡萄糖或蔗糖、1%蛋白胨或2%牛肉浸膏、0.02%K2HPO4、0.1%酵母膏。另外,添加硝态氮(KNO3)对2株固氮细菌的生长有明显的抑制作用,但氨态氮[(NH4)2SO4]则对细菌生长无影响。Isolates J115 and G161 of Enterobacter cloace(Jordan) Hormaeche and Edwards were two rice endophytic bacteria with associative nitrogen fixation.There was only little difference between the two isolates' nutrient requirement.The best formulation of the media was studied and determined,which was composed of 2% glucose or sucrose,1% peptone or 2% beef extract,0.02% K2HPO4 and 0.1% yeast extract.Furthermore,KNO3 had obvious inhibition to the bacterial growth,but(NH4)2SO4 had no influence.

关 键 词：水稻 内生细菌 联合固氮细菌 阴沟肠杆菌 营养 

分 类 号：S511[农业科学—作物学]