昆明山海棠总生物碱对人结肠癌HCT116细胞裸鼠皮下移植瘤生长的影响  

Effects of total alkaloids of tripterygium hypoglaucum hutch on the growth of xenografts of human colon cancer cell line HCT116 in nude mice

作　　者：黄晓春[1] 刘晋祎[1] 周燕虹[1] 敖琳[1] 杨录军[1] 曹佳[1] 

机构地区：[1]第三军医大学军事预防医学院军事毒理学教研室

出　　处：《免疫学杂志》2009年第5期547-549,553,共4页Immunological Journal

基　　金：重庆市自然科学基金(CSTC2009BB5026)

摘　　要：目的观察昆明山海棠总生物碱(total alkaloids oftripterygium hypoglaucum hutch,THHta)对人结肠癌HCT116细胞裸鼠皮下移植瘤生长的影响。方法18只Balb/c裸鼠建立人结肠癌HCT116细胞皮下移植瘤模型,瘤体积增至约100mm3时,随机分为对照组、THHta-1(100mg/kg)和THHta-2(200mg/kg)组,实验期间观测皮下移植瘤生长情况及裸鼠体重的变化,计算肿瘤相对增殖率及瘤重抑制率;取肿瘤组织及肝、脾、肾等行病理组织学检查;免疫组化法检测增殖细胞核抗原(proliferation cell nuclear antigen,PCNA)在肿瘤组织中的表达。结果THHta-1组和THHta-2组裸鼠肿瘤体积和重量显著降低,平均抑瘤率为60.4%和80.2%,治疗第21天两组相对肿瘤增殖率均<40%;THHta-1组和THHta-2组肿瘤组织中PCNA阳性细胞率显著低于对照组(P<0.01)。THHta致裸鼠体重减轻但裸鼠肝、脾、肾等组织未见明显病理学改变。结论THHta抑制人结肠癌HCT116细胞裸鼠皮下移植瘤生长,具有体内抗结肠癌活性,其作用可能与下调肿瘤组织PCNA表达有关。Objective To observe the effects of total alkaloids of tripterygium hypoglaucum hutch(THHta)on the growth of xenografts of human colon cancer cell line HCT116 in nude mice.Methods Nude mouse model bearing human colon cancer by subcutaneous transplantation of HCT116 cell line was reproduced.A total of 18 mice were randomly divided into 3 groups:control group,THHta-1(100 mg/kg)group and THHta-2(200 mg/kg)group.The growth of the xenografts and changes in mouse body weight in each group after treatment were mea...

关 键 词：昆明山海棠 生物碱 结肠癌 PCNA 

分 类 号：R735.35[医药卫生—肿瘤]