少突胶质谱系细胞易损性的研究  

A study of the vulnerability during the oligodendrocyte lineage cells development

作　　者：卢玉仙[1,2] 张海燕[2] 夏春林[2] 

机构地区：[1]盐城卫生职业技术学院护理系,盐城224005 [2]苏州大学医学部人体解剖学暨细胞神经生物学教研室

出　　处：《南通大学学报（医学版）》2009年第5期313-316,共4页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学基金资助项目(30772242)

摘　　要：目的:研究TNF-α对O-2A祖细胞、原少突胶质细胞、少突胶质细胞(oligodendrocytes,OL)这三个不同发育阶段少突胶质谱系细胞的易损性。方法:采用TNF-α分别刺激这三个不同发育阶段的细胞,MTT法检测存活率,免疫细胞化学检测活化的caspase-3。结果:O-2A祖细胞与OL相比存活率明显降低,原少突胶质细胞与OL相比存活率也降低;50ng/mlTNF-α刺激少突胶质谱系细胞48h以内,O-2A祖细胞的存活率与原少突胶质细胞、OL的存活率相比差异无统计学意义,而原少突胶质细胞的存活率与OL存活率相比较明显降低;caspase-3免疫细胞化学染色显示在这些细胞的胞浆和胞核有大量阳性信号。结论:TNF-α降低少突胶质谱系细胞的存活率具有细胞成熟程度的依赖性;caspase-3参与TNF-α诱导的3个不同发育阶段的OL的凋亡。Objecti ve:To study systematically the vulnerability of tumour necrosis factor-α(TNF-α) on developing oligodendrocyte lineage cells in culture.Methods:Oligodendrocyte lineage cells at three developmental stages were exposed to TNF-α , and then the viability of these cells was determined with MTT assay.The detection of apoptosis was performed with immuocytochemistry of caspase-3.Results:With the increase in TNF-α concentration, the survival rate of O-2A progenitor cells was decreased compared with oligodendr...

关 键 词：肿瘤环死因子α O-2A祖细胞 原少突胶质细胞 少突胶质细胞 易损性 

分 类 号：R741[医药卫生—神经病学与精神病学]