重组人RGD-FasL对胶质瘤细胞U138/U343/U373的体外抗肿瘤的活性分析  

Anti-tumor activities of recombined human RGD-FasL against three glioma cell lines U138/U373/U343

作　　者：罗芳洪[1] 李文珠[1,2] 丘劲华[1] 苏金华[1] 庄国洪[1] 

机构地区：[1]厦门大学医学院抗癌研究中心 [2]厦门大学生命科学学院

出　　处：《免疫学杂志》2009年第6期680-684,共5页Immunological Journal

摘　　要：目的研究重组人RGD-FasL对3株胶质瘤细胞U138/U343/U373的杀伤作用及机制。方法采用RT-PCR方法、MTT比色法、DNA倍体分析、流式细胞术检测融合蛋白对3株胶质瘤细胞的杀伤作用;利用Western blot法探讨其作用机制。结果FasmRNA在U138和U343细胞中有表达,在U373细胞中未有可见表达;DcR3mRNA在3株细胞中均有表达,在U373细胞中强表达。采用流式细胞术检测肿瘤细胞表面两种受体的表达情况,结果与RT-PCR相符。U138细胞株对RGD-FasL敏感并呈剂量依赖性;U343细胞株对RGD-FasL相对敏感;U373细胞对RGD-FasL不敏感。用PI检测细胞周期与凋亡表明RGD-FasL能使U138/U343细胞停留在G1期,推迟进入S期,抑制细胞生长并诱导细胞发生凋亡。Western blot实验表明RGD-FasL作用细胞后caspase-8/3/9的表达升高,Bcl-2的表达降低。结论重组人RGD-FasL可以不同程度的诱导胶质瘤细胞凋亡,其机制与其受体的表达和caspase-8/3/9、Bcl-2的表达有关。Objective To study the cytotoxic effects of RGD-FasL on glioma cell lines U138/U373/U343 and the underlying mechanism. Methods Fas/DcR3 mRNA was detected by RT-PCR and its surface expression in the three cell lines was measured by flow cytometry analysis. Cytotoxicity exerted by RGD-FasL on glioma cell lines was measured with MTT assay and the induced apoptosis was determined by agarose gel electrophoresis. The cell cycle and apoptosis were assessed by flow cytometry with PI staining. The expressions of Cas...

关 键 词：RGD-FasL 胶质瘤 靶向治疗 凋亡 

分 类 号：R739.4[医药卫生—肿瘤]