甲醛致胎鼠肝微核率及染色体畸变的研究  

Micronucleus rate and chromosome aberration of mouse embryo liver induced by formaldehyde

作　　者：曹霞飞[1] 曹智丽[1] 郑翔[1] 王穆[1] 李哲[1] 李程[1] 王婧[1] 龙鼎新[1] 让蔚清[1] 

机构地区：[1]南华大学生命科学研究中心环境医学与放射卫生研究所,衡阳421001

出　　处：《卫生研究》2009年第6期667-671,共5页Journal of Hygiene Research

基　　金：国家自然科学基金(No.30572191);南华大学博士启动基金(No.5-2005-XQD-001)资助

摘　　要：目的探讨甲醛致胎鼠肝微核率与染色体畸变。方法随机将妊娠小鼠(孕13天)分为5组:腹腔注射甲醛(0.00、0.20、2.00、20.00mg/kg)染毒组、环磷酰胺(30mg/kg)阳性对照组,实验至妊娠第14天即染毒后18小时脱颈椎处死孕鼠,剥离两侧子宫,每侧各取一胎鼠,断头放出外周血,取胎肝,采用胎肝微核试验和胎肝染色体畸变试验,检测胎肝血微核率和染色体畸变率。结果2.00、20.00mg/kg甲醛染毒组胎肝微核率及胎肝染色体畸变率与阴性对照组比较差异有显著性(P<0.01);染色体畸变主要表现为染色体断裂、多倍体等畸形。结论甲醛可致胎鼠肝微核率与染色体畸变。Objective To study the micronucleus rate and chromosome aberration of mouse embryo liver induced by formaldehyde.Methods The pregnant mice(been pregnant for 13 days)were divided into 5 groups randomly:Formaldehyde was given as intraperitoneal injection(0.00,0.20,2.00,20.00mg/kg)for exposure group and Cyclophosphamide(30mg/kg)for positive control group.When the 14th days(I.e After exposed in formaldehyde 18 hours),escapes the cervical vertebra cervical killed off pregnant mouse,peels on both sides of the ute...

关 键 词：甲醛 染色体畸变 微核率 孕鼠 

分 类 号：R114[医药卫生—卫生毒理学]