Noggin蛋白对D-半乳糖致衰老小鼠学习记忆及海马齿状回神经发生的影响  

Effect of intracerebroventricular injection of noggin on learning and memory and neurogenesis in hippocampal dentate gyrus of D-galactose induced aging mice

作　　者：朱俊德[1] 余资江[1] 康朝胜[1] 余彦[1] 

机构地区：[1]贵阳医学院基础医学院人体解剖学教研室,贵阳550004

出　　处：《山东大学学报（医学版）》2009年第8期72-74,78,共4页Journal of Shandong University:Health Sciences

基　　金：贵阳医学院博士启动基金资助项目(C2006-4);贵阳医学院青年教师基金资助项目(K2007-61)

摘　　要：目的观察侧脑室注射Noggin蛋白后对D-半乳糖致衰老模型小鼠学习记忆行为能力与海马齿状回神经发生的影响。方法采用皮下注射D-半乳糖构建衰老小鼠模型后,连续7 d侧脑室注射Noggin蛋白,对照组同时注射等量生理盐水,造模42 d后对各组小鼠进行Y迷宫学习记忆能力测试,用BrdU标记海马齿状回增殖细胞。结果Y迷宫检测结果表明,模型组小鼠学习记忆能力较正常对照组明显降低(P<0.05),而模型治疗组小鼠学习记忆能力较模型对照组明显改善(P<0.05);模型组与模型对照组小鼠海马齿状回BrdU阳性细胞数较正常对照组减少(P<0.05),而模型治疗组BrdU阳性细胞数较模型组、模型对照组显著增多(P<0.05)。结论侧脑室给予Noggin蛋白可改善衰老模型小鼠的空间学习及记忆能力,可能与Noggin能保护海马神经元并促使齿状回神经干细胞增殖有关。Objective To investigate endogenous neurogenesis and learning and memory in hippocampul dentate gyrus of D-galactose induced aging mice by intracerebroventricular injection of noggin protein.Method 40 mature KM male mice were randomly divided into the normal control group,the aging model group,the treatment control group and the noggin treatment group.In the aging model group,treatment control group and treatment group,D-galactose(125 mg·kg-1·d-1) was given to mice for 6 weeks by subcutaneous injection.Nogg...

关 键 词：半乳糖 海马 神经元 学习 记忆 Noggin蛋白 小鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]