SPK/S1P信号途径对人肝癌细胞VEGFmRNA表达的作用  

Role of the SPK/S1P signal on expression of VEGFmRNA in human hepatoma cells

作　　者：姜大磊[1] 高艳景[2] 刘慧亚[2] 

机构地区：[1]青岛市市立医院消化科,山东青岛260000 [2]山东大学齐鲁医院消化内科,济南250012

出　　处：《山东大学学报（医学版）》2009年第9期73-75,共3页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助课题(T2006C53)

摘　　要：目的探讨鞘氨醇激酶/1-磷酸鞘氨醇信号途径(SPK/S1P)在基因水平对人肝癌细胞分泌血管内皮生长因子(VEGF)的调节作用。方法体外培养人肝癌细胞HepG2,利用荧光定量PCR法检测鞘氨醇激酶的特异性抑制剂N,N′-二甲基鞘氨醇(DMS)作用后肝癌细胞VEGFmRNA的表达。结果不同浓度DMS处理后的HepG2细胞中VEGF基因表达较对照组明显降低,与对照组比较差异有统计学意义(P<0.01)。结论DMS可通过阻断SPK/S1P信号途径在基因水平抑制肝癌细胞VEGFmRNA,说明人肝癌细胞VEGFmRNA表达受SPK/S1P信号途径调控。Objective To investigate the role of the SPK/S1P(sphingosine kinase/sphingosine-1-phospate)signal on gene level in regulating expression of vascular endothelial growth factor(VEGF) in human hepatoma cells.Methods The human hepatoma cell line HepG2 was cultured in vitro.After treatment with a concentration of 520μmol/L DMS(dimethyl sphingosine) in culture media,VEGFmRNA expression was determined by RT-PCR.Results Expression of VEGFmRNA was obviously inhibited by DMS in a dose-dependent manner.Conclusion DMS ...

关 键 词：信号传导 癌 肝细胞 内皮 血管 生长物质 

分 类 号：R735.7[医药卫生—肿瘤]