TRAIL基因启动子区单核苷酸多态性与脂肪肝的相关性  

Relationship between single nucleotide polymorphism of TRAIL gene promoter region and fatty liver disease

作　　者：祁建妮[1] 阎晓华[2] 续力云[1] 赵培庆[1] 梁晓红[1] 马春红[1] 高立芬[1] 

机构地区：[1]山东大学医学院免疫研究所,济南250012 [2]山东医学高等专科学校,山东临沂276002

出　　处：《山东大学学报（医学版）》2009年第9期114-118,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助课题(30872298)

摘　　要：目的探讨中国山东汉族人群肿瘤坏死因子凋亡相关诱导配体(Tumor necrosis factor(TNF)-related apoptosis in-ducing ligand,TRAIL)基因启动子区单核苷酸多态性(single nucleotide polymorphism,SNP)与脂肪肝(FLD)的相关性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析和四引物法聚合酶链反应(Tetra-primer ARMS-PCR)的方法检测了42例非酒精性脂肪肝6、7例酒精性脂肪肝和132例健康对照者的TRAIL基因启动子区单核苷酸多态性位点-707C/T、-665T/C、-621C/T和-597A/G的基因型,计算相应的基因型频率及等位基因频率,进行χ2检验。结果-707C/T、-665T/C和-597A/G位点在中国山东汉族人群中未检测到单核苷酸多态性;-621C/T位点在中国山东汉族人群中存在单核苷酸多态性,且该位点CC/CT/TT基因型频率在非酒精性脂肪肝、酒精性脂肪肝以及正常对照组中分别为0.238/0.500/0.262、0.269/0.448/0.284和0.280/0.394/0.326。但该位点的基因型频率和等位基因频率在三组之间均没有统计学差异。结论在中国山东汉族人群中,仅检测到TRAIL基因启动子区-621C/T位点的单核苷酸多态性,该多态性与脂肪肝的易感性没有统计学差异(P>0.05)。Objective To investigate the distribution of single nucleotide polymorphism(SNP) in the promoter region of tumor necrosis factor-related apoptosis inducing ligand(TRAIL)gene and to evaluate the effect of SNP in the promoter region of the TRAIL gene and FLD susceptibility in the Chinese Han population from Shandong Province.Methods Genotype and allele frequencies of SNPs-707C/T,-665T/C,-621C/T and-597A/G in 42 and 67 non-or alcoholic fatty liver disease and 132 healthy controls were detected by polymerase ch...

关 键 词：肿瘤坏死因子 脂肪肝 多态性 单核苷酸 

分 类 号：R575.5[医药卫生—消化系统]