应用凝集素芯片检测胃正常黏膜上皮细胞与胃癌细胞膜表面糖链表达  

Glycan Profiling on the Cell Membrane of Normal Mucosal Epithelial Cells and Gastric Cancer Cells Detected with Lectin Array

作　　者：李春辉[1] 何群[1] 马静红[1] 潘忠诚[1] 王天骄[1] 张玉魁[1] 赵雨杰[1] 

机构地区：[1]中国医科大学生物芯片中心,卫生部细胞生物学重点实验室,沈阳110001

出　　处：《中国医科大学学报》2009年第11期807-809,813,共4页Journal of China Medical University

基　　金：国家自然科学基金资助项目(20672144)

摘　　要：目的应用凝集素芯片检测胃正常黏膜上皮细胞系GES-1和胃癌细胞系SGC7901、MKN45细胞膜表面糖链表达,并对芯片上捕获的细胞进行初步的免疫荧光研究。方法用胶原酶Ⅱ消化细胞系,利用芯片上的凝集素位点对糖链的亲和特异性捕获荧光标记的细胞,激光共聚焦扫描仪扫描检测细胞膜表面糖链表达。结果在大多数凝集素位点上,这几种细胞均显示出荧光信号;在凝集素位点WBA、EEL、MAH-Ⅱ上均未显示荧光信号;其中,与GES-1相比,胃癌细胞系SGC7901和MKN45在LTL、HHL位点上基本没有荧光信号。结论根据凝集素的亲和特异性说明,这几种细胞系膜表面共同表达的糖链包括:唾液酸、乙酰葡萄糖/葡萄糖、乙酰半乳糖/半乳糖、甘露糖等糖链。其中,胃癌细胞系SGC7901和MKN45可能较少表达岩藻糖。Objective To detect the glycan profiling on the cell membrane of normal mucosal epithelial cells and gastric cancer cell lines GES-1,SGC7901,and MKN45 with lectin array and immunofluorescence assay.Methods The cells were digested with collagenase Ⅱ.The labeled cells were caught by lectins printed on the array according to distinctive glycan-binding specificities.The glycan profiling was scanned by laser fluorescence scanner.Results All the cells presented binding signals on most of lectin-binding sites,exce...

关 键 词：凝集素芯片 胃癌 糖链表达 免疫荧光 

分 类 号：R735.2[医药卫生—肿瘤]