魔芋低聚糖硫酸酯的制备及抗病毒活性研究  

STUDY ON PREPARATION AND ANTIVIRUS ACTIVITY OF SULFATED AMORPHO PHALLUS KONJAC OLIGOSACCHARIDE

作　　者：杨敏[1] 蒙义文[1] 

机构地区：[1]中国科学院成都生物研究所,成都610041

出　　处：《天然产物研究与开发》2002年第1期17-20,共4页Natural Product Research and Development

基　　金：中国科学院知识创新工程西南基地项目;中国科学院九五重大项目专题 (编号 :KZ 95 1 A1 10 3 0 4 2 )

摘　　要：从酶解魔芋干粉中提取分子量在 15 0 0左右的葡甘露低聚糖Apkos ,采用吡啶 氯磺酸法制备了它的硫酸酯衍生物S Apkos,其硫含量为 8 2 8% ,取代度 (Ds)为 0 .5 7。用Vero细胞和单纯疱疹病毒 2型 (HSV 2 )考察硫酸酯化前后低聚糖的抗病毒活性。结果表明 ,本身无抗HSV 2活性的Apkos硫酸酯化后产生了抗病毒活性 ,5 0 0～ 40 0 0 μg/ml的S Apkos能很好地抑制HSV 2引起的Vero细胞病变 ,而在5 0 0 μg/ml及以上浓度条件下能完全抑制HSVApkos,a kind of oligosaccharide,was extracted from the cellulase enzymolysis product of Amorpho phallus konjac K.Koch.It weights about 1500,being composed of glucose and mannose.S-Apkos was prepared from Apkos by chemically modification with the method of chlorosulfonic acid-pyridine.The sulfur content of S-Apkos was found to be 8.28%.The average degree of substitution was 0.57.With the model of Vero cell and herpes simplex virus type 2(HSV-2),the antiviral activities of Apkos and S-Apkos were investigated in vitro.The results showed that Apkos itself did not inhibit the cytopathogenicity effect and plaque forming of HSV-2 in Vero cell.But its sulfated derivative obtained antiviral activity,which could well inhibit the cytopathogenicivty effect of HSV-2 at concentrations of 500～4000 μg/ml,and completely inhibit plaque forming of HSV-2 at concentrations above 500 μg/ml.

关 键 词：魔芋 低聚糖 硫酸酯 单纯疱疹病毒 

分 类 号：R93[医药卫生—生药学]