应用ATP生物荧光肿瘤体外药敏检测技术研究大肠癌药敏的异质性  

Heterogeneity of chemosensitivity of colorectal cancer determined by an ex vivo ATP-tumor chemosensitivity assay

作　　者：田海梅[1] 李艳芬[1] 吴健雄[2] 曹冬艳[1] 荣维淇[2] 刘毅[1] 李茉[1] 王海鹏[2] 王小兵[1] 张伟[1] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤医院肿瘤所生物检测中心,北京100021 [2]中国医学科学院中国协和医科大学肿瘤医院肿瘤所腹部外科,北京100021

出　　处：《癌症进展》2005年第5期436-441,共6页Oncology Progress

摘　　要：目的探讨用ATP生物荧光肿瘤体外药敏检测技术(ATP-TCA)研究大肠癌药敏的异质性和个体化疗的可行性。方法用ATP-TCA检测58例大肠癌标本对16种单药或联合用药的敏感性。结果个体之间的药物敏感性存在着明显的异质性。单药中最有效的药物为长春瑞滨、羟基喜树碱、氟尿嘧啶和紫杉醇。联合用药最有效的是氟尿嘧啶+丝裂霉素+阿糖胞苷,91.6%(11/12)的标本对其敏感,其次是氟尿嘧啶+顺铂+阿霉素,健择+顺铂和5-FU氟尿嘧啶+长春新碱+卡氮芥。结论大肠癌对抗癌药物的敏感程度存在着异质性。ATP生物荧光肿瘤药敏检测技术可用于为大肠癌选择合适的化疗药物。Objective To investigate the heterogeneity of chemosensitivity in colorectal cancer using an ATP-tumor chemosensitivity assay(ATP-TCA) and the feasibility of individual chemotherapy.Methods An ATP-TCA were used to determine the effect of 16 single or combined cytotoxic drugs in surgical specimens from 58 patients with colorectal cancer cancer.Results There were considerable differences in chemosensitivitvy between individuals.The most active single drugs in the assay was identified as novelbine,hydroxycamptothecin,5-fluorouracil and paclitaxel.5-fluorouracil+mitomycin+aytarabine was found to be the most effective combination in,for 91.6%(11/12)specimens were sensitive to this regimen.5-fluorouracil+cisplatin+adriamycin,gemcitabine+cisplatin and 5-fluorouracil +vincristine+carmustine were moderately active regi-mens.Conclusions There was the heterogeneity of the in vitro chemosensitivity in colorectal cancer.The use of the ATP-TCA provides a method of selecting appropriate anti-cancer drugs in colorectal cancer.

关 键 词：ATP生物荧光肿瘤药敏检测技术 大肠癌 

分 类 号：R73[医药卫生—肿瘤]