甲基叔丁基醚的遗传毒性研究  

Study on the Genotoxicity of Methyl Tertiary-Butyl Ether

作　　者：李红艳[1] 高锦伍[1] 杨江[1] 汪国雄[1] 倪春辉[1] 

机构地区：[1]东南大学公共卫生学院劳动卫生与环境卫生学系,南京210009

出　　处：《环境与职业医学》2001年第6期341-343,共3页Journal of Environmental and Occupational Medicine

基　　金：铁道部科技基金资助项目 (J98Z0 1 9)

摘　　要：[目的 ]探讨国产甲基叔丁基醚 (MTBE)的遗传毒性。 [方法 ]应用单细胞凝胶电泳试验、DNA交联试验和程序外DNA合成试验 ,测试MTBE对大鼠肺Ⅱ型细胞和肝细胞的遗传毒性。 [结果 ]MTBE可引起大鼠肺Ⅱ型细胞和肝细胞的DNA链断裂及程序外DNA合成的增加 ,不引起小牛胸腺DNA、大鼠肺Ⅱ型细胞DNA和肝细胞DNA交联。 [结论This study is to explore the genotoxicity of methyl tertiary buty1 ether(MTBE) produced in China. The in vitro genotoxicity of MTBE in rat type Ⅱ pneumocytes and hepatocytes was tested using single cell gel electrophoresis assay,DNA cross links test and unscheduled DNA synthesis assay. MTBE resulted in DNA strand breaks and increase of unscheduled DNA synthesis in both types of cells tested. It did not induce DNA cross links in calf thymus DNA,rat type Ⅱ pneumocyte DNA and rat hepatocyte DNA. [Conclusion] MTBE tested might have certain genotoxicity at DNA level.

关 键 词：甲基叔丁基醚 遗传毒性 单细胞凝胶电泳试验 DNA交联试验 程序外DNA合成试验 

分 类 号：R394.6[医药卫生—医学遗传学]