人大肠癌转移与Ⅳ型胶原酶的关系  

The Relationship of Metastasis with Type Ⅳ Collagenases in Human Colorectal Cancer

作　　者：蔡相军[1] 李京宁[1] 刘秋珍[1] 周晶[2] 段志泉[2] 

机构地区：[1]解放军第202医院普通外科,辽宁沈阳110003 [2]中国医科大学第一临床学院,辽宁沈阳110001

出　　处：《临床军医杂志》2000年第3期17-19,共3页Clinical Journal of Medical Officers

摘　　要：目的　探讨人大肠癌转移与Ⅳ型胶原酶的关系。方法　应用明胶酶谱分析法 (Zymography)检测了 31例大肠癌组织Ⅳ型胶原酶的活性 ,并用RT PCR方法检测 92kDⅣ型胶原酶RNA的表达情况。结果　在伴有转移的大肠癌标本中 ,Ⅳ型胶原酶的活性形式与非活性形式表达均较高 ,92kDⅣ型胶原酶的mRNA与在转移癌中高表达。结论　Ⅳ型胶原酶与大肠癌的转移密切相关。Objective To investigate the relationship of metastasis with type Ⅳ collagenases in human colorectal cancer Method Quantitatively detect activity of type Ⅳ collagenases by gelatinembedded SDS-PAGE and study 92 kD type Ⅳ collagenases gene expression by RT-PCR method in 31 cases of colorectal cancer Result The activity of both active and latent forms of type Ⅳ collagenases in metastatic cases is higher than that of of non-metastasis ones, and 92 kD type Ⅳ collagenase gene expression was also high in accordance with its activity Conclusion The metastasis of human colorectal cancer is closely related to type Ⅳ collagenases

关 键 词：Ⅳ型胶原酶 肿瘤转移 大肠肿瘤 

分 类 号：R735.34[医药卫生—肿瘤]