大肠癌动物模型的构建  

Model Building of Animals with Large Intestine Cancer

作　　者：聂春生[1] 赵玲辉[1] 薛红杰[2] 张慧博[1] 刘宝全[1] 

机构地区：[1]哈尔滨医科大学人体解剖学教研室,黑龙江哈尔滨150086 [2]哈尔滨医科大学附属第一医院第二住院部,黑龙江哈尔滨150001

出　　处：《第四军医大学吉林军医学院学报》2001年第1期23-24,共2页

摘　　要：目的　通过构建动物模型 ,研究大肠癌病灶区淋巴管及癌转移机制。方法　采用甲基硝基亚硝基胍(MNNG)直肠灌注法对Wistar大鼠每日灌肠 ,定期取材。结果　 16周HE染色可见局限粘膜层发生癌肿 ;2 2周可见粘膜下层癌肿 ;2 6周可见肌层内癌肿。结论　MNNG直肠灌注法诱发大肠癌成功率高 ,周期相对较短 ;Objective To research lymphatic vessels in focal area of large intestine cancer and mechanism of carcinometastasis.Methods By rectal perfusion,Wistar rats were given MNNG every day,and samples were collected periodically.Results Through HE staining,at the 16th week tumors were found limited in mucous layer,at the 22nd week in submucous layer and at 26th week in muscular layer.Conclusion: There is high rate success for MNNG to induce large intestine cancer with relatively short cycle and difficult oper...

关 键 词：大肠癌 动物模型 甲基硝基亚硝基胍 大鼠 

分 类 号：R735.34[医药卫生—肿瘤]