胞液型磷脂酶A_2cDNA与载体PRCCMV连接及表达  

Ligation and expression of cytosolic phospholipase A_2 cDNA and its carrier PRC/CMV

作　　者：刘华欣[1] 傅桂莲[2] 杜培革[2] 

机构地区：[1]第四军医大学吉林军医学院临床检验教研室,吉林吉林132013 [2]北华大学医学院中心实验室,吉林吉林132011

出　　处：《第四军医大学吉林军医学院学报》2001年第4期187-189,共3页

摘　　要：目的　选择适当的表达载体 ,启动胞液型磷脂酶A2 (cytosolicphospholipaseA2 ,简称cPLA2 )cDNA的表达 ,为进一步研究各种因素对该酶活性的影响奠定基础。方法　从cPLA2 PMT2 重组体中获得人cPLA2cDNA片段 ,使之与酶切线性化的PRC CMV载体平末端连接 ,并转染鼠巨噬细胞 ,筛选、扩增阳性细胞 ,提取RNA ,Northern杂交法检测cPLA2 mRNA。结果　应用cPLA2 PRC CMV重组体转染鼠巨噬细胞后 ,经过North ern杂交检测到cPLA2 cDNA的表达。结论　cPLA2 PRC CMV是一有效的表达系统。Objective To choose proper carriers for expression of cPLA 2 cDNA and lay a foundation for further research on the effect of many factors on the activity of enzyme.Methods From cPLA 2 PMT 2 recombinants,human cPLA 2 cDNA fragments were taken and ligated to PRC/CMV,then mouse macrophages were transfected for screening.Positive cells were increased and RNA was extracted,finally cPLA 2 mRNA was detected with northern blot.Results With cPLA 2 PRC/CMV combinant transfecting marcrophages,cPLA 2 mRNA e...

关 键 词：胞液型 磷脂酶A2 平末端连接 表达 

分 类 号：R[医药卫生]