黄荆子乙酸乙酯提取物对人肝癌裸鼠移植瘤生长的影响  

Effects of the Acetoacetate Extract of Vitex Negundo Seed on the Growth of Human Hepatocellular Carcinoma Xenograft in Nude Mice

作　　者：封萍[1] 周应军[2] 韩家凯[1] 庄英帜[1] 

机构地区：[1]南华大学附属第一医院肿瘤内科,湖南衡阳421001 [2]中南大学药学院,湖南长沙410013

出　　处：《湖南师范大学学报（医学版）》2008年第4期13-15,共3页Journal of Hunan Normal University(Medical Sciences)

基　　金：长沙市科技局科技计划重点项目(NO.KZB064003)

摘　　要：目的:观察黄荆子乙酸乙酯提取物(EVn-50)对人肝癌Hep G2裸鼠移植瘤生长的抑制作用。方法:采用人肝癌Hep G2裸鼠模型评估EVn-50在体治疗肝癌的有效性;免疫组化染色检测增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)、血管内皮生长因子(vascular endothelial growth factor,VEGF)、CD31蛋白表达。结果:EVn-50显著抑制人肝癌Hep G2裸鼠移植瘤的生长,呈剂量依赖性;5,10,20 mg/kg的EVn-50对移植瘤的瘤重抑制率分别为55%,62%,69%。免疫组化染色结果显示,EVn-50(5,10,20 mg/kg)与生理盐水组比较,PCNA、VEGF、CD31的表达下调,亦呈剂量依赖性。结论:EVn-50具有抑制人肝癌HepG2裸鼠移植瘤生长作用,其作用机制可能与其下调PCNA、VEGF、CD31的表达有关。Objective To investigate the effects of the acetoacetate extract of Vitex negundo seed(EVn-50) on the growth of human hepatocellular carcinoma xenograft in nude mice.Methods Human hepatocellular carcinoma xenograft in nude mouse model was established to evaluate the therapeutical efficacy of EVn-50 in vivo.The expression of proliferating cell nuclear antigen(PCNA),vascular endothelial growth factor(VEGF) and CD31 in the 20 xenograft specimens were detected by immunohistochemistry.Results Therapeutic trial o...

关 键 词：肝癌 黄荆子乙酸乙酯提取物 增殖细胞核抗原 血管内皮生长因子 CD31 

分 类 号：R735.7[医药卫生—肿瘤]