Large scale of identification of differentially expressed genes in the regenerating rat liver after SISPH  

作　　者：RAHMAN Salman 

机构地区：[1]Homophilia Research Center, London University, London SE17EH, United Kingdom

出　　处：《Science China(Life Sciences)》2005年第6期624-635,共12页中国科学（生命科学英文版）

基　　金：This work was supported by a grant from theNational Natural Science Foundation of China(Grant No.30270673);by a grant from the Zoological Key Subject ofHenan Province(Grant No.PKSO4004).

摘　　要：Extensive gene expression analysis was carried out after a 0, 4, 36, 72, 96 h short interval successive partial hepatectomy (SISPH) was performed. A total of 185 elements were identified as differing by more than two-fold in their expression levels at one or more time points. Of these 185 elements, 103 were up-regulated, 82 were down-regulated and 86 elements were unreported genes. Quite a few genes were previously unknown to be involved in liver regenera-tion (LR). Using cluster and general analysis, we found that the genes at five time points of the SISPH share eight different types of different expression profiles and eight distinct temporal in-duction or suppression patterns. A comparison of the gene expression in SISPH with that after PH found that 41 genes were specifically altered in SISPH, and 144 genes were simultaneously up-regulated or down-regulated in SISPH and after PH, but they were present in different amounts at the different time points. The conclusions are that (i) microarrays combined with suppressive subtractive hybridization (SSH) can effectively identify genes involved in LR on a large scale; (ii) more genes were up-regulated than down-regulated; (iii) there are fewer abun-dantly expressed genes than those with increased levels of 2-5 fold.Extensive gene expression analysis was carried out after a 0, 4, 36, 72, 96 h short interval successive partial hepatectomy (SISPH) was performed. A total of 185 elements were identified as differing by more than two-fold in their expression levels at one or more time points. Of these 185 elements, 103 were up-regulated, 82 were down-regulated and 86 elements were unreported genes. Quite a few genes were previously unknown to be involved in liver regenera-tion (LR). Using cluster and general analysis, we found that the genes at five time points of the SISPH share eight different types of different expression profiles and eight distinct temporal in-duction or suppression patterns. A comparison of the gene expression in SISPH with that after PH found that 41 genes were specifically altered in SISPH, and 144 genes were simultaneously up-regulated or down-regulated in SISPH and after PH, but they were present in different amounts at the different time points. The conclusions are that (i) microarrays combined with suppressive subtractive hybridization (SSH) can effectively identify genes involved in LR on a large scale; (ii) more genes were up-regulated than down-regulated; (iii) there are fewer abun-dantly expressed genes than those with increased levels of 2-5 fold.

关 键 词：PARTIAL HEPATECTOMY (PH)  short interval successive PARTIAL HEPATECTOMY (SISPH)  liver regeneration (LR)  subtracted cDNA libraries  complementary DNA microarray  cluster analysis. 

分 类 号：R575[医药卫生—消化系统]