核转录因子NF-κBp65、血管生成素-2和Ki-67在血管瘤组织中的表达及意义  

Expression of NF-κB p65,angiopoietin-2 and Ki-67 and its significance in proliferation and involuting period of hemangioma tissue

作　　者：毕庆伟[1] 赵雯雯[1] 包殿瑞[1] 刘树发[1] 

机构地区：[1]佳木斯大学口腔医院颌面外科,黑龙江佳木斯154002

出　　处：《黑龙江医药科学》2009年第5期22-23,共2页Heilongjiang Medicine and Pharmacy

摘　　要：目的:研究核转录因子NF-κB p65、血管生成素-2、Ki-67和CD31在血管瘤增生及退化组织中的表达情况。方法:采用免疫组织化学方法检测人皮肤血管瘤增生期、退化期及正常皮肤组织中核转录因子p65、血管生成素-2、CD31、Ki-67蛋白的表达水平。结果:增生期血管瘤内皮细胞CD31表达水平高于退化期,差异有显著性(P<0.05);增生期血管瘤内皮细胞核转录因子p65、血管生成素-2表达水平高于退化期及正常皮肤组,差异有显著性(P<0.05);退化期血管瘤内皮细胞核转录因子p65、血管生成素-2表达水平与正常皮肤组织相比,差异无显著性(P>0.05);核转录因子p65与血管生成素-2相关性分析在血管瘤发展过程中呈正相关(P<0.01);核转录因子p65与Ki-67相关性分析在血管瘤发展过程中呈正相关(P<0.01)。结论:在血管瘤组织中,核转录因子p65的表达与血管生成素-2和Ki-67的表达呈正相关,提示核转录因子p65可能通过正向调控血管生成素-2的表达而促进血管瘤的形成;核转录因子p65可能成为新的血管瘤病理分型标记物。Objective:To explore the expression of NF-κB p65,angiopoietin-2 and Ki-67 and its significance in the proliferation and involuting phases of hemangioma tissue.Methods:The expression of NF-κB p65,angiopoietin-2 and Ki67 was detected in 27 cases of the proliferation phase of hemangioma tissue and 15 of the involuting phase of hemangioma tissue by using immunohistochemical methods,while 5 cases of normal human skins were as controls.Results:The expression of NF-κB p65,angiopoietin-2,Ki-67 and CD31 were significant higher in the proliferation phase of hemangioma tissue than in the involuting phase of hemangioma tissue,respectively.There was no difference between the involuting phases of hemangioma tissue and normal skin tissue in the expression of either NF-κB p65 or angiopoietin-2,or Ki-67,or CD31.A positive correlation was found between NF-kB p65 v.s.angiopoietin-2 and NF-κB p65 v.s.Ki-67 in both the proliferation and involuting phases of hemangioma tissue(P<0.05).Conclusion:There is a positive correlation between the NF-κB p65 expression and the expression of angiopoietin-2 and Ki-67 in hemangioma tissue,which suggests that NF-κB p65 might promote the development of hemangioma through up-regulation of angiopoietin-2 expression.NF-κB p65 might become a new pathological type marker of hemangioma.

关 键 词：血管瘤 核转录因子p65 血管生成素-2 免疫组织化学 

分 类 号：R732.2[医药卫生—肿瘤]