肺癌抑癌基因1对前列腺癌T_3B细胞侵袭、成瘤和转移能力的影响  

Effect of TSLC1 Gene on Cell Aggression, Tumor Formation and Capability of Metastasis in Human Prostatic Carcinoma Cell Line T_3B

作　　者：袁林[1] 陈安民[1] 郭风劲[1] 祝文涛[1] 王江[1] 廖晖[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院,湖北武汉430030

出　　处：《肿瘤学杂志》2009年第11期990-994,共5页Journal of Chinese Oncology

基　　金：国家973重点基础研究发展规划资助项目(2002CB513107)

摘　　要：[目的]研究肺癌抑癌基因1(TSLC1)对人前列腺癌T3B细胞侵袭、成瘤和转移能力的影响。[方法]将克隆有TSLC1全长cDNA的真核表达载体pCI-TSLC1稳定转染至前列腺癌T3B细胞中。实验组T3B细胞转染pCI-TSLC1质粒,以转染空质粒pCI-neo的T3B细胞为对照组,未加任何处理的T3B细胞为空白组。Transwell法检测体细胞体外侵袭能力,将三组细胞分别以4.0×106/200μl浓度注入裸鼠皮下,观察各组成瘤情况,将三组细胞分别以2.0×106/10μl进行骨原位注射建立骨转移瘤模型,观察各组骨转移率。[结果]与对照组和空白组相比,实验组细胞株细胞体外侵袭能力受到显著抑制(P<0.01);实验组皮下瘤出现明显晚于对照组和空白组,而且瘤体也明显小于对照组和空白组(P<0.01);实验组骨转移率为20%,明显低于对照组(100%)和空白组(100%)(P<0.05)。[结论]TSLC1基因明显抑制T3B细胞的侵袭、成瘤和转移能力。[Purpose] To investigate the effects of tumor suppressor in lung cancer-1 (TSLC1) on cell aggression, tumor formation and capability of metastasis in human prostatic carcinoma cell line T3B. [Methods] Eukaryotic vector pCI-TSLC1 which had full length of TSLC1 cDNA were transfected into human prostatic carcinoma cell line T3B. The T3B cells transfected with this plasmid (experimental group) and those treated with pCI-neo vector (control group) and without any treatment (blank group) were compared. Cell aggre...

关 键 词：前列腺肿瘤 T3B细胞 抑癌基因 肺癌抑癌基因1 

分 类 号：R737.25[医药卫生—肿瘤]