PAK1基因对大肠癌细胞体外侵袭能力的影响  

Effect of p21-activated kinase-1 on the invasiveness of colorectal carcinoma cells in vitro

作　　者：武金宝[1] 韩宇晶[2] 南清振[1] 张振书[1] 张宏权[3] 宋于刚[1] 

机构地区：[1]南方医科大学南方医院消化病研究所,广东广州510515 [2]中山大学肿瘤研究中心,广东广州510060 [3]Karolinska Institute Department of Biosciences and Nutrition

出　　处：《南方医科大学学报》2009年第7期1341-1343,1347,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30171053)

摘　　要：目的研究p21-activated kinase-1(PAK1)基因对大肠癌细胞系体外侵袭能力的影响。方法把重组p21活化蛋白激酶1质粒用脂质体转染大肠癌SW480细胞,同时设立空白对照组和空载体对照组。于转染后48h采用免疫印迹方法检测PAK1的蛋白表达水平,Boyden小室模型检测大肠癌细胞SW480在转染重组PAK1基因质粒后侵袭能力的变化。结果SW480细胞转染p21活化蛋白激酶1重组质粒后,与空白对照和空载体对照相比,PAK1蛋白水平明显增加,细胞的体外侵袭能力增强。结论转染pPAK1重组质粒能够有效上调PAK1基因,增强大肠癌细胞系体外侵袭潜能,提示PAK1基因高表达可能与大肠癌细胞的侵袭和转移等生物学行为相关。Objective To observe the effect of p21-activated kinase-1(PAK1) gene transfection on the invasiveness of human colorectal carcinoma SW480 cells in vitro.Methods SW480 cells in routine culture were transfected with the recombinant plasmid EGFP-C1/PAK1 via LipofectamineTM 2000.The expression of PAK1 protein in SW480 cells was detected using Western blotting,and the changes of the invasiveness of SW480 cells were evaluated using Boyden chamber invasion assay.Results Forty-eight hours after transfection with pE...

关 键 词：p21-activated kinase-1 大肠癌 转移 

分 类 号：R735.3[医药卫生—肿瘤]