慢性肾衰营养不良大鼠血浆神经肽的表达及复方α-酮酸的干预  

Effects of α-keto acid on the expression of neuropeptide Y in malnutrition rats with chronic renal failure

作　　者：周伟东[1] 邓聪[2] 龙海波[1] 肖炜[3] 胡海燕[1] 

机构地区：[1]南方医科大学珠江医院肾内科,广东广州510282 [2]广州市第一人民医院南沙中心医院,广东广州510089 [3]南方医科大学中医药学院,广东广州510515

出　　处：《南方医科大学学报》2009年第7期1387-1389,共3页Journal of Southern Medical University

基　　金：广东省科技计划项目(2007B031500017)

摘　　要：目的观察慢性肾衰营养不良大鼠血浆神经肽(NPY)水平和下丘脑NPY基因表达,及复方α-酮酸对其的影响。方法5/6肾切除配合4%酪蛋白饮食制作慢性肾衰营养不良大鼠模型,造模成功后大鼠随机分为正常组、模型组、复方α-酮酸组。灌胃4周后放免法检测血浆NPY和半定量RT-PCR法检测下丘脑NPY基因表达。结果模型组血浆NPY显著升高,而下丘脑NPY基因表达显著下降,相关分析血浆NPY与下丘脑NPY呈显著负相关,复方α-酮酸组血浆NPY显著降低,NPY基因表达显著上升。结论慢性肾衰大鼠存在高NPY血症,NPY与慢性肾衰营养不良关系密切,复方α-酮酸可上调下丘脑NPY基因表达,降低血浆NPY水平,从而改善慢性肾衰营养不良。Objective To investigate the effects of α-keto acid on the expression of neuropeptide Y in malnutrition rats with chronic renal failure.Methods SD rats received 5/6 nephrectomy and were fed with 4% casein to establish models of malnutrition with chronic renal failure.Serum albumin,urea nitrogen,serum creatinine,type-1 insulin like growth factor and body weight of the rats were measured.The rat models were randomized into chronic renal failure group,α-keto acid group and normal control group,and after a 4-we...

关 键 词：慢性肾衰竭 营养不良 神经肽Y 复方Α-酮酸 

分 类 号：R692.5[医药卫生—泌尿科学]