高温复合脂多糖应激大鼠肾组织TNF-α表达变化  

Immunohistochemical study of TNF-α expression in the kidney of rats with lipopolysaccharide and heat stresses

作　　者：左丹[1] 周立宁[2] 谢峰[3] 何丹丹[4] 梅桂萍[4] 

机构地区：[1]中国人民解放军广州军区总医院骨科 [2]山东淄博市医疗保险事业处,山东淄博255047 [3]中国人民解放军广州军区总医院呼吸科 [4]中国人民解放军广州军区总医院护理部

出　　处：《南方医科大学学报》2009年第8期1629-1631,1634,共4页Journal of Southern Medical University

基　　金：中国人民解放军广州军区总医院刘坚院长基金

摘　　要：目的探讨高温与脂多糖(LPS)复合应激大鼠肾组织TNF-α的表达特点。方法雄性SPF级Wistar大鼠随机分为常温+生理盐水组(C组)、高温+生理盐水组(H组)、常温+LPS组(L组)、高温+LPS组(HL组)。置动物于模拟气候舱,HL组、H组暴露环境干球温度(dry bulb temperature,Tdb)为(35.0±0.5)℃,L组和C组Tdb为(26±0.5)℃;HL组和L组动物经尾静脉ivLPS10mg/kg,H组和C组动物经尾静脉iv9g/LNaCl10ml/kg。应激120min时,免疫组织化学SABC染色法检测动物肾组织TNF-α的表达特点,并做常规病理学检查。结果高温与LPS复合应激组肾组织TNF-α的表达显著增强,肾损伤程度加重。结论高温与LPS复合应激造成的肾毒性与肾TNF-α表达密切相关。Objective To investigate the effects of co-exposure of LPS and heat on TNF-α expression in rat kidneys. Methods Male pathogen-free Wistar rats were randomly assigned in saline-injected normothermic control (C group), saline-injected heat exposure (H group), LPS-injected normothermic control (L group), and LPS-injected heat exposure (HL group). The rats in H and HL groups were exposed in a chamber at an ambient dry bulb temperature (Tdb) of 35.0±0.5 degrees, and those in C and L groups were exposed to a Tdb ...

关 键 词：热应激 脂多糖 免疫组化 肿瘤坏死因子-Α 

分 类 号：R363[医药卫生—病理学]