家兔脊髓缺血再灌注损伤后内皮细胞功能和血小板活化状态的动态研究  

Changes of endothelial cell function and platelet activation in rabbit spinal cord with ischemia-reperfusion injury

作　　者：巩守平[1] 王文涛[1] 钟大林[1] 吕健[1] 吴锋[2] 车金[2] 僧志远[1] 贺西京[3] 

机构地区：[1]西安交通大学医学院第二附属医院神经外科,陕西西安710004 [2]西安交通大学医学院血液病实验室,陕西西安710061 [3]西安交通大学,陕西西安710004

出　　处：《南方医科大学学报》2009年第8期1638-1640,共3页Journal of Southern Medical University

摘　　要：目的探讨内皮细胞功能和血小板活化状态在脊髓缺血再灌注损伤(SCI/RI)中的意义。方法采用Zivin法建立家兔SCI/RI模型,动态观察SCI/RI前后血浆一氧化氮(NO)和血小板α颗粒膜蛋白140(GMP140)的变化。结果NO在I/R组缺血末期显著上升(P<0.01),I/R2h达到峰值,与sham组比较有显著意义(P<0.01);I/R6h下降但仍有显著差异(P<0.05);GMP-140在sham组无显著变化,在I/R组缺血末期显著升高(P<0.01),I/R2h后逐渐下降到正常水平。结论血浆NO和GMP140在SCI/RI后显著升高,表明内皮细胞受刺激/损伤和血小板过度活化参与了脊髓I/R损伤与修复病理过程。Objective To study the changes of vascular endothelial cell function and platelet activation in rabbit spinal cord following ischemia-reperfusion (I/R) injury and their roles in the spinal cord injury. Methods Rabbit spinal cord I/R injury models were established using Zivin method, and the changes in plasma NO and GMP140 levels were dynamically monitored after the injury. Results Plasma NO level increased significantly in the I/R group at the end of the ischemia, and reached the peak level at 2 h of reperf...

关 键 词：脊髓 缺血再灌注 内皮细胞功能 血小板活化 一氧化氮 α颗粒膜蛋白140 

分 类 号：R363[医药卫生—病理学]