小檗碱对大鼠结肠上皮细胞钙依赖钾通道的影响  

Effects of berberine on basolateral calcium-activated potassium current of rat colonic crypt cells

作　　者：尹琬凌[1] 吴杰[1] 王萍[1] 罗和生[2] 

机构地区：[1]武汉市中心医院消化内科,湖北武汉430014 [2]武汉大学人民医院消化内科

出　　处：《胃肠病学和肝病学杂志》2009年第11期1022-1024,共3页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的研究小檗碱对结肠上皮隐窝细胞基底膜钙依赖钾通道(IK(Ca))的影响,以探讨其治疗分泌性腹泻的机制。方法用EDTA溶液分离结肠上皮隐窝细胞,运用EPC10膜片钳放大器测量全细胞模式下50、100、500μmol/L的小檗碱对结肠上皮细胞基底膜IK(Ca)的影响。结果50、100、500μmol/L的小檗碱均可抑制大鼠结肠上皮隐窝细胞基底膜IK(Ca)(P<0.05),当阶跃刺激为+80 mV时,其IK(Ca)分别为生理盐水(PSS)对照组的(71.43±3.61)%、(54.56±5.13)%、(38.66±3.85)%(n=8,P<0.05)。结论小檗碱能抑制大鼠结肠上皮细胞基底膜钙依赖钾通道的开放,这可能是其治疗分泌性腹泻的机制之一。Objective To clarify the mechanism of berberine on secretory diarrhea by investigating the effects of berberine on basolateral calcium-activated potassium current(IK(Ca)) of the rat colonic crypt cells. Methods The intact colonic crypt cells were isolated by the EDTA solution.The effects of berberine on IK(Ca) were detected by patch clamp technique,under the conventional whole cell patch clamp mode. Results 50,100,500 μmol/L berberine inhibited IK(Ca) of rat colonic crypt cells significantly(P<0.05).When de...

关 键 词：小檗碱 结肠上皮隐窝细胞 膜片钳 钙依赖钾通道 

分 类 号：R574.62[医药卫生—消化系统]