结肠炎诱导大鼠行为学变化及骶髓后连合核和丘脑板内核群中GFAP表达的意义  

The changes of behavior and expressions of GFAP in dorsal commissural nucleuse and thalamic intralaminar nuclei in rats induced by colitis

作　　者：王景杰[1] 杨琦[1] 秦明[1] 王旭霞[1] 卢王[1] 张超[1] 黄裕新[1] 

机构地区：[1]第四军医大学唐都医院消化内科,陕西西安710038

出　　处：《胃肠病学和肝病学杂志》2009年第11期1028-1032,共5页Chinese Journal of Gastroenterology and Hepatology

基　　金：国家自然科学基金项目(No30871142)

摘　　要：目的探讨因福尔马林引起的大鼠急性结肠炎刺激诱导的行为学变化及骶髓后连合核(dorsal commissural nucleuse,DCN)和丘脑板内核群(thalamic intralaminar nuclei,ILN)胶质原纤维酸性蛋白(glial fibrillary acidic protein,GFAP)的表达及其意义。方法给大鼠乙状结肠注射5%的福尔马林100μL制作急性结肠炎引发内脏痛大鼠模型。采用痛行为学积分方法观察不同时间点急性结肠炎大鼠行为学变化。采用免疫组织化学方法,观察不同时间点急性结肠炎大鼠骶髓后连合核和丘脑板内核GFAP表达变化情况。结果急性结肠炎大鼠痛行为表现高峰为造模后60 min,90 min时减低程度显著。GFAP在骶髓后连合核的表达在造模形成后的45 min最为明显,在90 min明显降低。而在丘脑板内核的表达60 min最为明显,120 min明显降低。结论大鼠痛行为学的变化是由于急性炎症引起的内脏痛而导致,与疼痛变化密切相关的胶质细胞中GFAP在脊髓内反应早且明显,而在与情绪变化密切相关的丘脑内的板内核群中变化相对较晚。Objective To study the changes of behavior and expressions of glial fibrillary acidic protein(GFAP) in dorsal commissural nucleuse(DCN) and thalamic intralaminar nuclei(ILN) in formalin induced acute colitis in rats.Methods An animal model of acute colitisin visceralgia rats was used in this study.The model was established by injection 100 μL of 5% formalin into sigmoid colon of rats.Pain score,behavioral changes were observed at different time points.Immunohistochemisty stain was used to assess the express...

关 键 词：结肠炎 福尔马林 内脏痛 GFAP 

分 类 号：R574.62[医药卫生—消化系统]