耗氧率及溶氧胁迫对长蛸体内酶活力的影响  

Oxygen Consumption Rate and Effect of Hypoxia Stress on Enzyme Activity of Octopus variabilis

作　　者：张晓梅[1] 王春琳[1] 李来国[1] 陈武[1] 

机构地区：[1]宁波大学省部共建"应用海洋生物技术"教育部重点实验室,浙江宁波315211

出　　处：《水生态学杂志》2010年第2期72-79,共8页Journal of Hydroecology

基　　金：国家十一五科技支撑计划(2007BAD43B05)资助

摘　　要：采用Winkler法测定水中溶氧含量,通过比较对照呼吸室与试验呼吸室水中溶解氧含量之差,测定长蛸(Octopus variabilisSasaki)耗氧率及窒息点,并在不同程度的溶氧胁迫下测定长蛸体内多种酶的活力变化。结果表明,长蛸耗氧率在一个昼夜内呈现'低-高-低-高'的趋势;耗氧率与水温(10~30℃)呈正相关,与pH值(6.40~9.20)呈负相关;随着盐度的升高(14~31),长蛸耗氧率表现为两头高、中间平稳,曲线呈凹状分布;雌性长蛸耗氧率高于雄性。窒息点随长蛸体重的增加而降低,平均体重为(30.50±6.03)g的长蛸窒息点为0.4179mg/L;平均体重为(54.50±4.65)g的长蛸窒息点为0.3902mg/L;平均体重为(97.00±11.36)g的长蛸窒息点为0.2738mg/L。随着溶氧胁迫程度的增加,LDH活力呈先升后降趋势,脂肪酶活力下降,SOD、POD、CAT、ACP、AKP和蛋白酶活力均呈'降-升-降'的趋势。The oxygen consumption rate and the suffocation point of Octopus variabilis were determined by comparing the dissolved oxygen content of control and experimental respiration chambers while the dissolved oxygen was measured based on the Winkler s Method.The changes of enzyme activity under the different levels of hypoxia stress were studied.The results indicated that the oxygen consumption rate of Octopus variabilis exhibited an obvious fluctuation of down-up-down-up in day and night,and they were positive...

关 键 词：长蛸 耗氧率 窒息点 溶氧胁迫 酶活力 

分 类 号：S917.4[农业科学—水产科学]