Androgen receptor coregulator ARA267-α interacts with death receptor-6 revealed by the yeast two-hybrid  

作　　者：MAI Tiejun WANG Xin ZHANG Zhiwen XIN Dianqi NA Yanqun GUO Yinglu 

机构地区：[1]Institute of Urology,First Hospital of Peking University,Beijing 100034,China [2]Department of General Surgery,First Hospital of Peking University,Beijing 100034,China [3]Department of Physiology,Peking University,Beijing 100083,China

出　　处：《Science China(Life Sciences)》2004年第5期442-448,共7页中国科学(生命科学英文版)

基　　金：This work was supported by National Nature Science Foundation of China(Grant No.30120007);the National Special Fund for“211 Project”of China(Grant No.217).

摘　　要：ARA267-a is a newly identified androgen receptor coactivator. In order to further elucidate its precise role in cells, using the ARA267- a fragment containing four PHD and one SET conserved domains as bait we revealed an ARA267-a-PHD-SET-interacting protein, death receptor-6 (DR6), in the yeast two-hybrid screening. DR6 is the member of TNF receptor family and has a death domain in its intracellular cytoplasmic portion (DR6cp) to mediate the cell apoptosis. The interaction between ARA267-a-PHD-SET and DR6cp was confirmed in vitro and in vivo. Our finding implied that androgen signaling pathway might cross talk with apoptosis sig-naling pathway through the interaction between ARA267-a and DR6.ARA267-a is a newly identified androgen receptor coactivator. In order to further elucidate its precise role in cells, using the ARA267- a fragment containing four PHD and one SET conserved domains as bait we revealed an ARA267-a-PHD-SET-interacting protein, death receptor-6 (DR6), in the yeast two-hybrid screening. DR6 is the member of TNF receptor family and has a death domain in its intracellular cytoplasmic portion (DR6cp) to mediate the cell apoptosis. The interaction between ARA267-a-PHD-SET and DR6cp was confirmed in vitro and in vivo. Our finding implied that androgen signaling pathway might cross talk with apoptosis sig-naling pathway through the interaction between ARA267-a and DR6.

关 键 词：ARA267-a androgen receptor androgen receptor coregulator death receptor-6 yeast two-hybrid system 

分 类 号：R363[医药卫生—病理学]