氟苯尼考对大黄鱼的急性毒性及体外抑菌试验  

Experimental Study on Inhibiting Bacteriain Vitro and Acute Toxicity of Florfenicol to Pseudosciaena crocea

作　　者：李思源[1] 邢晨光[1] 李凯[2] 吕永林[2] 王国良[1] 

机构地区：[1]宁波大学生命科学与生物工程学院,浙江宁波315211 [2]温州市渔业技术推广站,浙江温州325003

出　　处：《水生态学杂志》2010年第3期132-136,共5页Journal of Hydroecology

基　　金：农业部公益性行业科研专项(200903029);浙江省科技厅重点攻关项目(2005C23080);温州市科技局科技兴海项目(S20060039)

摘　　要：采用倍比稀释法,研究氟苯尼考对哈氏弧菌、副溶血弧菌、溶藻弧菌等8种大黄鱼(Pseudosciaena crocea)致病弧菌的体外抑菌效应;同时以棋盘微量稀释法进行五倍子、黄连、乌梅与氟苯尼考的联合用药试验,并研究了该药对养殖大黄鱼的急性毒性效应。结果表明,氟苯尼考对所选弧菌的最小抑菌浓度(MIC)在0.39～0.78μg/mL,且其MBC值与MIC值相等或为对倍关系;联合用药显示,MIC值比单独使用时略有降低,FIC指数为1.00～0.75,其中以氟苯尼考和五倍子的协同作用最为显著;氟苯尼考口服给药对大黄鱼具有较高安全性,96h LD50>1000mg/kg,对大黄鱼的安全浓度SC>100mg/kg。The checkerboard microdilution method was applied to study the effect of florfenicol on inhibiting bacteriain in vitro isolated from diseased Pseudosiaena crocea with disk-diffusion method. It is studied that the effect of florfenicol combined with 3 Chinese herbal medicines and florfenicol with Pseudosciaena crocea. The results showed that MIC values of florfenicol against eight strains of vibrios were in the range 0. 39-0. 78 μg/mL ,and the minimal bactericidal concentrations(MBC) was equal or doubled to ...

关 键 词：氟苯尼考 大黄鱼 体外抑菌 急性毒性 

分 类 号：S948[农业科学—水产养殖]