灿烂弧菌灭活菌苗对美国红鱼免疫效果的研究  

Study on Immune Efficacy Induced by Killed Vibrio splendidus Vaccine on Sciaenops ocellatus

作　　者：马爱敏[1] 闫茂仓[1] 胡利华[1] 谢起浪[1] 陈少波[1] 单乐州[1] 

机构地区：[1]浙江省海洋水产养殖研究所浙江省近岸水域生物资源开发与保护重点实验室,浙江温州325005

出　　处：《水生态学杂志》2010年第3期137-141,共5页Journal of Hydroecology

基　　金：浙江省科技厅科研院所青年人才计划项目(2006R20001);浙江省科技厅科研院所专项公益技术攻关项目(2007F10009);温州市科技兴海项目(S20070051)联合资助

摘　　要：制备灿烂弧菌(Vibrio splendidus)福尔马林灭活菌苗,通过浸泡、注射和口服3种方式接种美国红鱼(Sci-aenops ocellatus),分别在接种免疫后第7、14、21、28、35天采鱼血,检测其血清抗体效价、溶菌酶活力和白细胞吞噬活性;第28天用1.0×108CFU/mL的灿烂弧菌悬液进行攻毒试验,检测菌苗的免疫效果。结果表明,各免疫组血清抗体效价在第21天达到峰值,且浸泡组和注射组的血清抗体效价高于口服组;在35d实验期内,各免疫组的血清溶菌酶活力有明显提高,各浸泡组和注射组于免疫接种后14d显著高于对照组(P<0.05);除浸泡3组外,其余各免疫组的白细胞吞噬百分比(PP)和吞噬指数(PI)在免疫接种后14d均较对照组有显著差异(P<0.05);各组受免鱼对人工攻毒均具有保护作用,以注射2组的免疫保护率最高,达77.8%。Sciaenops ocellatus were vaccinated with a formalin-killed Vibrio splendidus vaccine via immersion,injection and oral route,respectively. Antibody titers,lysozymes,leucocyte phagocytosis were examined on the 7th, 14th,21st,28th d after first-lvaccination. A dosage of 0. 2 mL of V. splendidus 1. 0 × 108 CFU/mL was intraperitoneally injected to the vaccinated fish to evaluate the protective efficiency of the vaccine. The results indicated that: the antibody titers of all immunized group was the highest on the...

关 键 词：美国红鱼 灿烂弧菌 菌苗 

分 类 号：S941[农业科学—水产养殖]