新型环氧水解酶手性拆分环氧氯丙烷的气相色谱法  

Separation and determination of chiral epichlorohydrin by gas chromatography with a new epoxy hydrolase

作　　者：卢定强[1,2] 涂清波[1] 凌岫泉[1] 王俊[1] 党安旺[1] 李衍亮[1] 陈佳[1] 常亚军[1] 

机构地区：[1]南京工业大学药学院,南京210009 [2]江苏省药物研究所,南京210009

出　　处：《分析试验室》2010年第4期6-8,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20876076);国家重点基础研究发展计划(2009CB724700)项目资助

摘　　要：建立了一种用于快速测定生物酶转化体系中手性环氧氯丙烷的气相色谱法。采用ZKAT-ChiralB毛细管柱,FID检测器检测,内标法计算含量。(R)-环氧氯丙烷和(S)-环氧氯丙烷在0.012～0.461g/mL呈良好的线性关系,RSD为1.3%、1.7%,回收率为97.6%、101.3%。A method for determining chiral epichlorohydrinin in the system of enzymatic transformation by gas chromatography was developed.The chiral epichlorohydrinin was analyzed by gas chromatography with ZKAT-Chirl B capillary column and flameionization detection using an internal standard method.(R)-epichlorohydrin and (S)-epichlorohydrin showed a good linear relationship in the range from 0.012 to 0.461 g/mL.The precision test show that the RSDs were 1.3% and 1.7%,and the recoveries were 97.6% and 101.3%.

关 键 词：气相色谱 酶转化 手性 环氧氯丙烷 

分 类 号：TQ223.26[化学工程—有机化工]