豇豆花叶病毒和黑眼豇豆花叶病毒RT-Realtime PCR及IC-RT-Realtime PCR检测方法研究  

Research on RT-Realtime PCR and IC-RT-Realtime PCR detection of Cowpea mosaic virus and Blackeye cowpea mosaic virus

作　　者：李彬[1] 粟寒[1] 李艳华[2] 缪爱斌[3] 吴翠萍[1] 安榆林[1] 

机构地区：[1]江苏出入境检验检疫局,江苏南京210001 [2]无锡出入境检验检疫局,江苏无锡214101 [3]南京出入境检验检疫局,江苏南京211106

出　　处：《南京农业大学学报》2010年第2期105-109,共5页Journal of Nanjing Agricultural University

基　　金：江苏检验检疫局科研项目(2006KJ35)

摘　　要：建立了豇豆两种种传病毒豇豆花叶病毒(CPMV)和黑眼豇豆花叶病毒(BlCMV)RT-Realtime PCR检测方法以及双重RT-Realtime PCR检测方法。在此基础上,根据免疫学抗原抗体特异性吸附的特点,建立了CPMV和BlCMV免疫捕获反转录荧光定量(IC-RT-Realtime)PCR检测方法以及双重IC-RT-Realtime PCR检测方法。该单重及双重IC-RT-Real-time PCR检测方法具有灵敏度高、特异性强和稳定性好等优点,适用于豆类种苗中对CPMV和BlCMV的检测。The methods of using RT-Realtime PCR and dual RT-Realtime PCR to detect the two seed-borne viruses Cowpea mosaic virus(CPMV)and Blackeye cowpea mosaic virus(BlCMV)were established on cowpea.Furthermore,integrating specific absorbance characteristic of antigen and antibody,single and dual immuno-capture(IC)-RT-Realtime PCR methods for detecting CPMV and BlCMV were optimized.IC-RT-Realtime PCR showed high sensitivity,strong specificity and good stability.The study showed that the single and dual IC-RT-Realtim...

关 键 词：豇豆花叶病毒 黑眼豇豆花叶病毒 免疫捕获反转录实时荧光PCR 双重反转录实时荧光PCR 

分 类 号：S436.43[农业科学—农业昆虫与害虫防治]