Expression of interleukin 6 in brain and colon of rats with TNBS-induced colitis  

作　　者：Wang, Kai Yuan, Chuan-Ping Wang, Wei Yang, Zhan-Qing Cui, Wei Mu, Lian-Zhi Yue, Zhan-Peng Yin, Xiu-Ling Hu, Zhong-Ming Liu, Ju-Xiong 

机构地区：[1]Jilin Univ, Coll Anim Sci & Vet Med, Jilin Prov Key Lab Anim Embryo Engn, Changchun 130062, Jilin Province, Peoples R China [2]Univ Hebei No, Dept Vet Med, Zhangjiakou 075029, Hebei Province, Peoples R China [3]Acad Mil Med Sci, Beijing 100071, Peoples R China

出　　处：《World Journal of Gastroenterology》2010年第18期2252-2259,共8页世界胃肠病学杂志（英文版）

基　　金：Supported by The grant from the National Natural Science Foundation of China,No 30871840;No 30901057

摘　　要：AIM:To characterise expression of interleukin 6(IL-6),a potent proinflammatory cytokine,in the occurrence and development of inflammatory bowel disease(IBD) and investigate its effect on neuroimmunomodulation and immune homeostasis regulation.METHODS:In this study,rats with colitis induced by trinitrobenzene sulfonic acid(TNBS) were sacrificed on days 3,7,14,21 and 28 after induction.In the controls,the TNBS was just replaced by equivalent amount of phosphate buffered solution(PBS,0.01 mol/L).IL-6 mRNA expression in brain and colon tissues in each phase was evaluated by real-time reverse transcriptionpolymerase chain reaction,and cellular localisation and protein level of IL-6 was determined by immunohistochemistry.RESULTS:At day 7,mRNA expression of IL-6 was significantly higher in the colon and brain of IBD rats than that of the controls.The protein level was also significantly higher in colon,hypothalamus and cerebral cortex of IBD rats compared with the controls.So there are similar temporal trends in IL-6 mRNA expression and protein levels in all positions with a persistent increase to a peak at day 7,followed by a decline and gradual return to normal levels.CONCLUSION:These results revealed that changes in IL-6 expression in brain and colon tissues occur in different phases of IBD.Therefore,we propose that the nerve centre regulates and controls the occurrence and development of IBD via IL-6.AIM:To characterise expression of interleukin 6(IL-6),a potent proinflammatory cytokine,in the occurrence and development of inflammatory bowel disease(IBD) and investigate its effect on neuroimmunomodulation and immune homeostasis regulation.METHODS:In this study,rats with colitis induced by trinitrobenzene sulfonic acid(TNBS) were sacrificed on days 3,7,14,21 and 28 after induction.In the controls,the TNBS was just replaced by equivalent amount of phosphate buffered solution(PBS,0.01 mol/L).IL-6 mRNA expr...

关 键 词：Inflammatory bowel disease Interleukin 6 NEUROIMMUNOMODULATION Real-time transcription-polymerase chain reaction Immunohistochemistry 

分 类 号：R574.62[医药卫生—消化系统]