Nardostachys jatamansi extract protects against cytokine-induced β-cell damage and streptozotocin-induced diabetes  

作　　者：Mi-Young Song Ui-Jin Bae Bong-Hee Lee Kang-Beom Kwon Eun-A Seo Sung-Joo Park Min-Sun Kim Ho-Joon Song Keun-Sang Kwon Jin-Woo Park Do-Gon Ryu Byung-Hyun Park 

机构地区：[1]Department of Biochemistry, Medical School and Diabetes Research Center, Chonbuk National University, Jeonju, Jeonbuk 561-756, South Korea [2]Department of Physiology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, South Korea [3]Department of Food and Nutrition, School of Human Environmental Science, Wonkwang University, Iksan, Jeonbuk 570-749, South Korea [4]Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, South Korea [5]Department of Preventive Medicine, Medical School, Chonbuk National University, Jeonju, Jeonbuk 561-756, South Korea

出　　处：《World Journal of Gastroenterology》2010年第26期3249-3257,共9页世界胃肠病学杂志（英文版）

基　　金：Supported by Grant from the Ministry of Science and Technology/Korea Science and Engineering Foundation through the Diabetes Research Center at Chonbuk National University, R13-2008-005-0000-0;a Research Fund of Chonbuk National University in 2009 (to Park BH)

摘　　要：AIM: To investigate the anti-diabetogenic mechanism of Nardostachys jatamansi extract (NJE). METHODS: Mice were injected with streptozotocin viaa tail vein to induce diabetes. Rat insulinoma RINm5F cells and isolated rat islets were treated with interleukin1β and interferon-γ to induce cytotoxicity. RESULTS: Treatment of mice with streptozotocin resulted in hyperglycemia and hypoinsulinemia, which was conf irmed by immunohistochemical staining of the islets. The diabetogenic effects of streptozotocin were completely abolished when mice were pretreated with NJE. Inhibition of streptozotocin-induced hyperglycemia by NJE was mediated by suppression of nuclear factor (NF)-κB activation. In addition, NJE protected against cytokine-mediated cytotoxicity. Incubation of RINm5F cells and islets with NJE resulted in a signif icant reduction in cytokine-induced NF-κB activation and downstream events, inducible nitric oxide synthase expression and nitric oxide production. The protective effect of NJE was further demonstrated by the normal insulin secretion of cytokine-treated islets in response to glucose. CONCLUSION: NJE provided resistance to pancreatic β-cell damage from cytokine or streptozotocin treatment. The β-cell protective effect of NJE is mediated by suppressing NF-κB activation.AIM: To investigate the anti-diabetogenic mechanism of Nardostachys jatamansi extract (NJE). METHODS: Mice were injected with streptozotocin viaa tail vein to induce diabetes. Rat insulinoma RINm5F cells and isolated rat islets were treated with interleukin1β and interferon-γ to induce cytotoxicity. RESULTS: Treatment of mice with streptozotocin resulted in hyperglycemia and hypoinsulinemia, which was conf irmed by immunohistochemical staining of the islets. The diabetogenic effects of streptozotocin were c...

关 键 词：Nardostachys jatamansi CYTOKINES STREPTOZOTOCIN Pancreatic β cells Nuclear factor κB Nitric oxide Diabetes mellitus 

分 类 号：R587.1[医药卫生—内分泌]