抑制survivin表达增强人肝癌HepG2细胞对高线性能量转移射线的辐射敏感性  

Inhibiting Survivin Expression Increases The Radiosensitivity of Human Hepatoma HepG2 Cells to High-LET Radiation

作　　者：金晓东[1] 李强[1] 李萍[1,2] 吴庆丰[1,2] 陶家军[1,2] 郝冀方[1] 戴中颖[1,2] 刘新国[1,2] 

机构地区：[1]中国科学院近代物理研究所,兰州730000 [2]中国科学院研究生院,北京100039

出　　处：《生物化学与生物物理进展》2010年第1期79-84,共6页Progress In Biochemistry and Biophysics

基　　金：国家高技术研究发展计划(863)资助项目(2006AA02Z499);国家自然科学基金重点资助项目(10835011);国家自然科学基金资助项目(10905080);中国科学院西部之光资助项目(0962030XBO)~~

摘　　要：探讨了肿瘤细胞中survivin的表达对高线性能量转移(LET)射线辐射敏感性的影响.根据Gen Bank提供的survivin序列,合成特异性survivin-siRNA寡核苷酸,转染人肝癌HepG2细胞,抑制survivin的表达.发现siRNA转染后诱导了HepG2细胞G2/M期阻滞,增加了自发性和辐射诱导的细胞凋亡.在高线性能量转移(LET)碳离子辐照后,siRNA转染细胞的克隆存活率明显下降.这些结果表明survivin表达是HepG2细胞产生对高LET射线辐射抗性的关键因素.The influence of survivin expression on the radiosensitivity of tumor cells to high linear energy transfer (LET) radiation is investigated. Survivin-specific short-interfering RNA (siRNA) oligonucleotides were synthesized based on the survivin sequence provided by GenBank. Human hepatoma HepG2 cells were transfected with survivin-specific siRNA to inhibit its expressions. It was found that the transfection with surviving-specific siRNA increased the levels of G2/M arrest and the apoptotic rates induced by r...

关 键 词：SURVIVIN 高射线能量转移(LET)射线 SIRNA 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]