利用酿酒酵母转座子文库筛选线粒体镁代谢相关基因  

A Transposon-Based Genetic Screen in Saccharomyces cerevisiae Reveals a Role of YMR166C in Mitochondrial Magnesium Homeostasis

作　　者：王娟[1] 范强旺[1] 周兵[1] 

机构地区：[1]清华大学生物科学与技术系,北京100084

出　　处：《生物化学与生物物理进展》2010年第1期36-41,共6页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目(30871262)~~

摘　　要：镁离子对于维持细胞正常功能十分重要,糖尿病、高血压、慢性呼吸道疾病、骨质疏松、心律失常等多种疾病都与镁代谢失衡有关.MRS2基因编码线粒体镁离子转运蛋白,MRS2缺失会导致酵母线粒体镁离子浓度下降、线粒体内Ⅱ型内含子剪接缺陷和非发酵碳源培养基上的生长缺陷.为了增进对线粒体镁离子代谢调控基因的了解,利用酿酒酵母mTn-lacZ/LEU2转座子文库筛选MRS2的抑制基因,发现线粒体载体家族成员YMR166C基因的缺失可以挽救MRS2基因缺失突变体的生长缺陷、Ⅱ型内含子剪接缺陷,并可以调节线粒体镁离子浓度,首次发现YMR166C是线粒体镁代谢相关基因.Magnesium homeostasis is very important to normal functions of cell and plays a role in a number of diseases such as diabetes, hypertension, chronic respiratory disorder, osteoporosis and arrhythmia. MRS2 encode a magnesium transporter protein in Saccharomyces cerevisiae and deletion of MRS2 gene results in decrease in mitochondrial magnesium concentration, group Ⅱ RNA splicing defect and growth defect on nonfermentable carbon source. To obtain more information of magnesium homeostasis in mitochondria, mTn-...

关 键 词：酵母 筛选 镁 线粒体 

分 类 号：Q78[生物学—分子生物学]