Effect of charge at an amino acid of basic fibroblast growth factor on its mitogenic activity  

作　　者：Wu, Xiao Ping Li, Xiao Kun 

机构地区：[1]Jinan Univ, Inst Tissue Transplantat & Immunol, Guangzhou 510632, Guangdong, Peoples R China [2]Jinan Univ, Natl Engn Res Ctr Gene Med, Guangzhou 510632, Guangdong, Peoples R China

出　　处：《Chinese Chemical Letters》2010年第4期468-471,共4页中国化学快报（英文版）

基　　金：supported by grants from the Natural Science Foundation of China(No.30973671);the Natural Science Foundation of Guangdong Province of China(No.9151064001000031)

摘　　要：The amino acid at the 119th position of human basic fibroblast growth factor(hbFGF),lysine(K119),is a critical component for its mitogenic activity.However,little is known about the effects of the characteristics of this residue including charge on the mitogenic activity of hbFGF.Herein,this basic residue was replaced with neutral glutamine residue and acidic glutamic acid residue to construct mutants hbFGF^(K119Q) and hbFGF^(K119E),respectively.The mutants were produced by BL21(DE3)/pET3c expression system...The amino acid at the 119th position of human basic fibroblast growth factor(hbFGF),lysine(K119),is a critical component for its mitogenic activity.However,little is known about the effects of the characteristics of this residue including charge on the mitogenic activity of hbFGF.Herein,this basic residue was replaced with neutral glutamine residue and acidic glutamic acid residue to construct mutants hbFGF^(K119Q) and hbFGF^(K119E),respectively.The mutants were produced by BL21(DE3)/pET3c expression system...

关 键 词：Human basic fibroblast growth factor Amino acid charge EXPRESSION Mitogenic activity Erk1/2 activation 

分 类 号：O621.2[理学—有机化学]